Hypercarb 5µm 50x2.1mm Column

The Hypercarb HPLC column 50x2.1mm, 5µm, based on porous graphitic carbon with a homogeneous graphitic surface without bonded ligands. Retention combines dispersive, electronic and pronounced shape-selective interactions. The chemically robust phase retains very polar, isomeric and ionic substances that are only weakly separated on conventional reversed phases. Hypercarb is used particularly for carbohydrates, glycan isomers, polar metabolites, nucleosides and structural isomers.

Features and Benefits

• Porous graphitic carbon as the stationary phase
• High retention of polar and structurally similar compounds
• Shape and charge selectivity in addition to hydrophobicity
• Broad pH and solvent stability

Additional Notes

Hypercarb is not based on bound silica, but entirely on porous graphitic carbon. The planar, highly polarizable surface generates hydrophobic, dispersive, charge-induced, and shape-selective interactions. As a result, highly polar or structurally similar molecules are often retained more strongly than on C18. When developing methods, eluent strength, temperature, and the specific adsorption characteristics of graphite must be taken into account.

Legal Information

Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. Hypercarb refers to a product line within the Thermo Scientific chromatography portfolio.