Acclaim Polar Advantage II 5µm 250x21.2mm

The Acclaim PolarAdvantage II (PA2) HPLC column 250x21.2mm, 5µm, based on 120Å silica with a polar embedded amide phase. The reversed-phase combines C18-like retention with polar selectivity and stable wetting. A wide usable pH range and low secondary interactions support robust methods for ionisable compounds. Acclaim PolarAdvantage II (PA2) is used particularly for basic active substances, polar metabolites, organic acids and general RP-HPLC.

Features and Benefits

• Selectivities for RP, HILIC, mixed-mode, and SEC
• Phases for general and application-specific LC methods
• Silica and polymer materials
• Analytical, UHPLC, and semipreparative formats

Additional Notes

The Acclaim product family includes classic reverse-phase, polar embedded, and HILIC phases, multimodal materials, and application-optimized columns for ions, surfactants, explosives, carbonyls, carbamates, and organic acids. The selection depends on the analyte’s polarity, charge state, desired retention mechanism, and detection method. For mixed-mode columns, the pH value, buffer type, ionic strength, and organic content must be optimized together; SEC ranges are always calibration-dependent reference values.

Legal Information

Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. Acclaim refers to a product line within the Thermo Scientific chromatography portfolio.