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The Waters Sep-Pak Silica SPE cartridge is based on unmodified silica and has a polar silanol surface. Chromatographically the Waters Sep-Pak Silica is designed for application in normal-phase SPE. The silica surface adsorbs polar analytes from non-polar solvents and is suitable for classical adsorption cleanup protocols. Applications of the Waters Sep-Pak Silica include lipids, pigments, natural products, extract cleanup and normal-phase fractionation.
Sep-Pak includes classic silica-based SPE phases as well as inorganic adsorbents and derivatized specialty cartridges. C18, tC18, C8, CN, NH2, and Diol cover different polarity and selectivity ranges. Silica, alumina, and Florisil operate primarily through adsorption mechanisms, whereas DNPH-Silica chemically derivatizes and subsequently retains carbonyl compounds. The wide selection of cartridge sizes allows sample volume, sorbent quantity, and required capacity to be tailored to the specific workflow.
Sep-Pak is a registered trademark of Waters Corporation, Milford, Massachusetts, USA.
| Brand | Sep-Pak |
|---|---|
| Product Type | SPE Cartridges |
| Particle Size | 55-105 µm |
| Chemistry | Silica |
| Chemistry Producers Term | Silica |
| Pore Size | 125 Ã… |
| Bet Surface Area [m2/g] | 325.0 |
| Country of Origin | United States of America (USA) |
| Bed Mass [mg] | 500 mg |
| pH Minimum | 0.0 |
| pH Maximum | 8.0 |
| Mode of Separation | Normal Phase Chromatography (NP) |
| Matrix | Silica |
| Particle Morphology | fully porous |