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The DNASep Ultra Capacity HPLC column 50x4.6mm utilises C18-alkylated, non-porous PS/DVB microspheres with markedly increased binding capacity. DNASep Ultra Capacity combines the series' typical DNA selectivity with a loading of up to approximately 1.7 mg total DNA or 300 µg crude DNA. The column is suitable for DNA purifications and fractionations; crudely purified plasmid DNA or kit-prepared samples can be injected directly with an appropriate matrix.
The DNASep series is based on a polystyrene-divinylbenzene polymer matrix rather than a silica backbone. This makes DNASep particularly suitable for aqueous-organic ion-pair methods in nucleic acid chromatography. The non-porous, high-performance PS/DVB particle structure supports rapid mass transfer and reproducible separations of DNA fragments. In practical applications, the product line is primarily used for dsDNA and ssDNA analyses, DNA calibration, mutation detection, fragment size determination, and DNA purification.
DNASep is a brand name of the manufacturer ADS Biotec Inc., Omaha, USA.
| Brand | DNASep |
|---|---|
| Product Type | HPLC Column |
| Length | 50 mm |
| Internal Diameter | 4.6 mm |
| Pore Size | non-porous |
| Country of Origin | United States of America (USA) |
| Column Type | Analytical Column |
| Base Material | Styrene-divinylbenzene |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| Mode of Separation | Reversed Phase Chromatography (RP) |
| Technology | HPLC |
| Matrix | Polymer |