OligoPrep™ HC3, alkylated 9um, porous SS 7.8mmx150mm

The OligoPrep HC3 HPLC column (150 × 7.8 mm, 10 µm) is based on alkylated, porous polystyrene-divinylbenzene particles for preparative ion-pair reverse-phase chromatography. OligoPrep HC3 is designed for higher loadings and is used for the purification of synthetic oligonucleotides, DNA fragments and related nucleic acids. Thanks to the macroporous polymer matrix, the phase can also accommodate larger oligonucleotide structures and elute them reproducibly. The OligoPrep HC3 column is therefore particularly suitable for scale-up steps following analytical screening, for fractionation and for preparative HPLC methods using TEAA or comparable buffer systems.

Features and Benefits

• For preparative oligo purification
• Alkylated PS/DVB particles
• Macroporous matrix
• High binding capacity
• Ion-pair RP-HPLC

Additional Information

OligoPrep is designed for the preparative and semipreparative purification of synthetic oligonucleotides. The columns are based on an alkylated, macroporous polystyrene-divinylbenzene matrix. Thanks to the larger pore structure and the longer column format, larger sample volumes can be processed than with purely analytical OligoSep variants. The product line is typically operated with ion-pair buffer systems and an organic modifier to separate and purify oligonucleotides, DNA fragments, or related nucleic acid products.

Legal Information

OligoPrep is a brand name of the manufacturer Concise Separations, San Jose, USA.