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The Waters Spherisorb SCX HPLC column 250x4.6mm, 10µm, is based on spherical silica and carries strong cation-exchange groups. The Waters Spherisorb SCX is used for cation-exchange chromatography and separates analytes via charge-dependent interactions. The strong cation-exchange functionality provides retention for basic or positively charged analytes. In practice the Waters Spherisorb SCX is suitable, for example, for basic pharmaceuticals, biogenic amines, ionic compounds and methods with charge selectivity.
The Spherisorb series is based on mechanically robust silica with a pore size of 80 Å and is available in several particle sizes. ODS1 has a lower ligand density and lower carbon loading, while ODS2 offers a more densely packed, end-capped C18 surface. The series is complemented by short alkyl chains, cyano, phenyl, amino, and silica phases, as well as strong anion- and cation-exchange phases. This allows for the coverage of a wide range of classical HPLC methods using a silica base that is consistent across the product family.
Spherisorb is a registered trademark of Waters Corporation, Milford, Massachusetts, USA.
| Brand | Spherisorb |
|---|---|
| Product Type | HPLC Cartridge Column |
| Length | 250 mm |
| Internal Diameter | 4.6 mm |
| Particle Size | 10 µm |
| Chemistry | Strong cation-exchange |
| Chemistry Producers Term | SCX |
| Pore Size | 80 Å |
| Carbon Load | 4.0 % |
| Bet Surface Area [m2/g] | 220.0 |
| Country of Origin | United States of America (USA) |
| Hardware Material | Stainless Steel |
| Hardware Type | Cartridges |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Mode of Separation | Ion Exchange Chromatography |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |