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The Waters Spherisorb ODSB HPLC cartridge column 150x4.6mm, 5µm, is based on an octadecylsilyl phase (other designations: ODS, RP-18, C18) on spherical 80Å silica and is used in reversed-phase chromatography. Characteristic of Spherisorb ODSB are the base-deactivated, strongly endcapped surface and a medium ligand density with approximately 11.5% carbon loading. This surface chemistry reduces unwanted silanol interactions and promotes symmetrical peaks particularly for basic analytes. The Waters Spherisorb ODSB is used, for example, for pharmaceuticals, environmental methods such as EPA 535 and existing procedures where the specific ODSB selectivity is specified. For analytical ODSB cartridge columns the Waters End Connector Kit WAT037525 is required.
The Spherisorb series is based on mechanically robust silica with a pore size of 80 Å and is available in several particle sizes. ODS1 has a lower ligand density and lower carbon loading, while ODS2 offers a more densely packed, end-capped C18 surface. The series is complemented by short alkyl chains, cyano, phenyl, amino, and silica phases, as well as strong anion- and cation-exchange phases. This allows for the coverage of a wide range of classical HPLC methods using a silica base that is consistent across the product family.
Spherisorb is a registered trademark of Waters Corporation, Milford, Massachusetts, USA.
| Brand | Spherisorb |
|---|---|
| Product Type | HPLC Cartridge Column |
| Length | 150 mm |
| Internal Diameter | 4.6 mm |
| Particle Size | 5 µm |
| Chemistry | C18 (RP-18, ODS, Octadecyl) |
| Chemistry Producers Term | ODSB |
| Pore Size | 80 Å |
| USP Class | L1 |
| Carbon Load | 11.5 % |
| Bet Surface Area [m2/g] | 220.0 |
| Endcapping | endcapped |
| Country of Origin | United States of America (USA) |
| Hardware Material | Stainless Steel |
| Hardware Type | Cartridges |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Mode of Separation | Reversed Phase Chromatography (RP) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |