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The Waters Spherisorb Cyano HPLC column 100x4.6mm, 5µm, is built on spherical silica and features a cyanopropyl modification. The principal application of the Waters Spherisorb Cyano lies in normal phase and reversed-phase chromatography. The cyano group combines low hydrophobicity with dipolar interactions. In practice, the Waters Spherisorb Cyano is suitable, for example, for polar small molecules, normal phase methods, RP alternative selectivity and validation of older methods.
The Spherisorb series is based on mechanically robust silica with a pore size of 80 Å and is available in several particle sizes. ODS1 has a lower ligand density and lower carbon loading, while ODS2 offers a more densely packed, end-capped C18 surface. The series is complemented by short alkyl chains, cyano, phenyl, amino, and silica phases, as well as strong anion- and cation-exchange phases. This allows for the coverage of a wide range of classical HPLC methods using a silica base that is consistent across the product family.
Spherisorb is a registered trademark of Waters Corporation, Milford, Massachusetts, USA.
| Brand | Spherisorb |
|---|---|
| Product Type | HPLC Column |
| Length | 100 mm |
| Internal Diameter | 4.6 mm |
| Particle Size | 5 µm |
| Chemistry | CN (Cyano, Nitril) |
| Chemistry Producers Term | Cyano (CN) |
| Pore Size | 80 Å |
| USP Class | L10 |
| Carbon Load | 3.0 % |
| Bet Surface Area [m2/g] | 220.0 |
| Endcapping | endcapped |
| Country of Origin | United States of America (USA) |
| Column Type | Analytical Column |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Mode of Separation | Semipolar Phase Chromatography |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |