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The Waters Spherisorb Amino HPLC column 250x10mm, 10µm, is based on spherical silica and features an aminopropyl modification. The Waters Spherisorb Amino is intended for use in normal phase, HILIC and weak anion exchange chromatography. The amino group enables polar and ionic interactions and is particularly suitable for carbohydrates. Typical applications of the Waters Spherisorb Amino include sugar analysis, polar natural products, normal-phase methods and weakly acidic analytes.
The Spherisorb series is based on mechanically robust silica with a pore size of 80 Å and is available in several particle sizes. ODS1 has a lower ligand density and lower carbon loading, while ODS2 offers a more densely packed, end-capped C18 surface. The series is complemented by short alkyl chains, cyano, phenyl, amino, and silica phases, as well as strong anion- and cation-exchange phases. This allows for the coverage of a wide range of classical HPLC methods using a silica base that is consistent across the product family.
Spherisorb is a registered trademark of Waters Corporation, Milford, Massachusetts, USA.
| Brand | Spherisorb |
|---|---|
| Product Type | HPLC Column |
| Length | 250 mm |
| Internal Diameter | 10 mm |
| Particle Size | 10 µm |
| Chemistry | NH2 (Amino) |
| Chemistry Producers Term | Amino (NH2) |
| Pore Size | 80 Å |
| USP Class | L8 |
| Carbon Load | 1.9 % |
| Bet Surface Area [m2/g] | 220.0 |
| Endcapping | non endcapped |
| Country of Origin | United States of America (USA) |
| Column Type | Semi-preparative Column |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Mode of Separation | Semipolar Phase Chromatography |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |