SEC-GPC

Size exclusion chromatography (SEC), also known as gel permeation chromatography (GPC) and gel filtration chromatography (GFC), is a chromatographic process in which analytes are separated according to size and structure. It is used to characterise natural and synthetic polymers, biopolymers, proteins and nanoparticles.

The largest molecules are the first to leave the separation column. The smaller the molecules are, the better they can penetrate the pores of the stationary phase and the later they are eluted from the column (see illustration).

Schematic function of the size exclusion mechanism

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Toyopearl HW-55S, 30 µm, 500 Å 250 mL

TSH-07459

Product Type: SEC / GPC Bulk Amount: 250 ml Particle Size: 30 µm Pore Size: 500 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer pH Minimum: 2.0 pH Maximum: 13.0

Toyopearl HW-55F, 45 µm, 500 Å 500 mL

TSH-07457

Product Type: SEC / GPC Bulk Amount: 500 ml Particle Size: 45 µm Pore Size: 500 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer pH Minimum: 2.0 pH Maximum: 13.0

Toyopearl HW-50S, 30 µm, 125 Å 250 mL

TSH-07455

Product Type: SEC / GPC Bulk Amount: 250 ml Particle Size: 30 µm Pore Size: 125 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer pH Minimum: 2.0 pH Maximum: 13.0

Toyopearl HW-50F, 45 µm, 125 Å 500 mL

TSH-07453

Product Type: SEC / GPC Bulk Amount: 500 ml Particle Size: 45 µm Pore Size: 125 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer pH Minimum: 2.0 pH Maximum: 13.0

Toyopearl HW-40S, 30 µm, 50 Å 250 mL

TSH-07451

Product Type: SEC / GPC Bulk Amount: 250 ml Particle Size: 30 µm Pore Size: 50 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer

Toyopearl HW-40C, 75 µm, 50 Å 500 mL

TSH-07449

Product Type: SEC / GPC Bulk Amount: 500 ml Particle Size: 75 µm Pore Size: 50 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer

Toyopearl HW-40F, 45 µm, 50 Å 500 mL

TSH-07448

Product Type: SEC / GPC Bulk Amount: 500 ml Particle Size: 45 µm Pore Size: 50 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer pH Minimum: 2.0 pH Maximum: 13.0

TSKgel HXL-L Guardcolumn, 7 µm, for G1000HXL-G4000HXL and GMHXL-L 40 mm x 6.0 mm

TSH-07113

Product Type: SEC / GPC Guard Columns Length: 40 mm Internal Diameter: 6.0 mm Particle Size: 7 µm USP Class: L21 Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Column Type: Analytical Column Base Material: Styrene-divinylbenzene Mode of Separation: SEC / Organic Size Exclusion Chromatography (GPC) Hardware Type: Ready-to-use Column Matrix: Polymer Technology: HPLC

TSKgel SW Top-Off, 10 µm, for all 7,5mm ID SW 1.0 g wet gel

TSH-06819

Product Type: SEC / GPC Bulk Particle Size: 10 µm USP Class: L20, L33, L59 Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Silica

TSKgel PWL Guardcolumn, 13 µm, for G1000PW and G2000PW 75 mm x 7.5 mm

TSH-06763

Product Type: SEC / GPC Guard Columns Length: 75 mm Internal Diameter: 7.5 mm Particle Size: 13 µm USP Class: L25, L39 Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Column Type: Analytical Column Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Hardware Type: Ready-to-use Column Matrix: Polymer Particle Morphology: spherical pH Minimum: 2.0 pH Maximum: 12.0 Technology: HPLC

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SEC
MZ-Gel SDplus GPC Columns Brochure 2020

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