SEC-GPC

Size exclusion chromatography (SEC), also known as gel permeation chromatography (GPC) and gel filtration chromatography (GFC), is a chromatographic process in which analytes are separated according to size and structure. It is used to characterise natural and synthetic polymers, biopolymers, proteins and nanoparticles.

The largest molecules are the first to leave the separation column. The smaller the molecules are, the better they can penetrate the pores of the stationary phase and the later they are eluted from the column (see illustration).

Schematic function of the size exclusion mechanism

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Toyopearl HW-40C, 75 µm, 50 Å 1 liter

TSH-14683

Product Type: SEC / GPC Bulk Amount: 1 l Particle Size: 75 µm Pore Size: 50 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer

Toyopearl HW-40F, 45 µm, 50 Å 1 liter

TSH-14682

Product Type: SEC / GPC Bulk Amount: 1 l Particle Size: 45 µm Pore Size: 50 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer pH Minimum: 2.0 pH Maximum: 13.0

Toyopearl HW-40S, 30 µm, 50 Å 1 liter

TSH-14681

Product Type: SEC / GPC Bulk Amount: 1 l Particle Size: 30 µm Pore Size: 50 Å Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer

TSKgel HXL-H Guardcolumn, 13 µm, for G5000HXL-G7000HXL + GMHXL 40 mm x 6.0 mm

TSH-13727

Product Type: SEC / GPC Guard Columns Length: 40 mm Internal Diameter: 6.0 mm Particle Size: 13 µm USP Class: L21 Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Column Type: Analytical Column Base Material: Styrene-divinylbenzene Mode of Separation: SEC / Organic Size Exclusion Chromatography (GPC) Matrix: Polymer Particle Morphology: non-porous Technology: HPLC

TSKgel SW Guardcolumn Glass, 10 µm, for all 8 mm ID SW and QC-PAK Glass 40 mm x 8.0 mm

TSH-08805

Product Type: SEC / GPC Guard Columns Length: 40 mm Internal Diameter: 8.0 mm Particle Size: 10 µm USP Class: L20, L33, L59 Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Column Type: Semi-preparative Column Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Hardware Type: Ready-to-use Column Matrix: Silica Technology: HPLC

TSKgel G4000SW Glass, 13 µm, 450 Å 300 mm x 8.0 mm

TSH-08801

Product Type: SEC / GPC Columns Length: 300 mm Internal Diameter: 8.0 mm Particle Size: 13 µm Pore Size: 450 Å USP Class: L20, L33, L59 Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Column Type: Semi-preparative Column Lower Molecular Weight: 2.000 (PEG/PEO), 4.000 (Dextran), 20.000 (Protein) Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Hardware Type: Ready-to-use Column Matrix: Silica Pressure Stability: 20 bar Technology: HPLC Upper Molecular Weight: 250.000 (PEG/PEO), 500.000 (Dextran), 7.000.000 (Protein)

TSKgel G3000SW Glass, 10 µm, 250 Å 300 mm x 8.0 mm

TSH-08800

Product Type: SEC / GPC Columns Length: 300 mm Internal Diameter: 8.0 mm Particle Size: 10 µm Pore Size: 250 Å USP Class: L20, L33, L59 Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Column Type: Semi-preparative Column Lower Molecular Weight: 1.000 (PEG/PEO), 2.000 (Dextran), 10.000 (Protein) Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Hardware Type: Ready-to-use Column Matrix: Silica Pressure Stability: 20 bar Technology: HPLC Upper Molecular Weight: 35.000 (PEG/PEO), 70.000 (Dextran), 500.000 (Protein)

TSKgel SWXL Top-Off, 5 µm, for SWXL and QC-PAK g wet gel

TSH-08544

Product Type: SEC / GPC Guard Kits Particle Size: 5 µm USP Class: L20, L33, L59 Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Silica Technology: HPLC

TSKgel SWXL Guardcolumn, 7 µm, for SWXL and P/N 16215 and 16049 40 mm x 6.0 mm

TSH-08543

Product Type: SEC / GPC Guard Columns Length: 40 mm Internal Diameter: 6.0 mm Particle Size: 7 µm USP Class: L20, L33, L59 Manufacturer: Tosoh Bioscience Delivery Time: on stock Column Type: Analytical Column Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Silica Technology: HPLC

TSKgel PWXL Top-Off, 10 µm, for all PWXL and G-DNA-PW 1.0 g wet gel

TSH-08035

Product Type: SEC / GPC Guard Kits Particle Size: 10 µm USP Class: L39 Manufacturer: Tosoh Bioscience Delivery Time: approx. 4 days Base Material: Hydroxylated polymethacrylate Mode of Separation: SEC / Aqueous Size Exclusion Chromatography (GFC) Matrix: Polymer Particle Morphology: spherical pH Minimum: 2.0 pH Maximum: 12.0 Technology: HPLC

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SEC
MZ-Gel SDplus GPC Columns Brochure 2020

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