ACE 1.7 Extended Method Development UHPLC/HPLC Column Kit 50x3.0mm (SuperC18 C18-Amide AND CN-ES)

The Avantor ACE C18-300 HPLC column 50x3.0mm, 1.7µm, is a wide-pore phase with 300 Å pores and an octadecylsilyl modification (other designations: ODS, RP-18, C18) for reversed-phase chromatography of larger molecules. The Avantor ACE C18-300 facilitates access of larger or bulky analytes to the C18 surface and thereby supports reproducible retention of biomolecules. The wide pore structure is particularly relevant when proteins or peptides can only partially diffuse into the pore structure of 100 Å materials. Typical applications are proteins, peptides, angiotensin peptides, insulin analogues, trastuzumab, oligomers and high-molecular-weight pharmaceutical samples.

Performance Characteristics and Benefits

• Ultra-inert, high-purity, base-deactivated silica particles
• Broad range of selectivity for reverse-phase, normal-phase, and HILIC applications
• Fully porous 100 Å and 300 Å phases for small molecules and biomolecules
• Excellent peak shapes for acidic, basic, and neutral analytes
• High reproducibility from column to column and batch to batch

Additional Information

The Avantor ACE Series is based on high-purity, spherical silica with very low metal contamination and targeted base deactivation. Controlled surface chemistry reduces the activity of free silanol groups, which promotes symmetrical peaks and reproducible retention times, particularly for basic analytes. The portfolio includes classic fully porous 100 Å phases, alternative selectivities, and 300 Å wide-pore materials for larger molecules. Using compatible Avantor ACE guard cartridges protects the silica backbone of the analytical column from matrix components and can extend the column’s service life.

Legal Notices

Avantor ACE is a registered trademark of the manufacturer Avantor, Inc., Radnor, USA.