SMT-Separation Methods Technologies / SMT SAM

SMT SAM

SMT SAM columns from SMT are based on the self-assembled monolayer (SAM) technique. This bonding technique allows ordered monolayers of functional groups. This is also called "horizontal polymerisation", due to Si-O-Si bonds running parallel to the silica surface. As a result, a higher ligand density can be achieved compared to conventional "vertical polymerization".

This SAM technology is available for reversed phase, ion exchange, affinity, chiral chromatography as well as for UHPLC.

Schematic representation of the SMT SAM modification of SMT

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WCX-10-100/25 Ion-Exchange WCX Column Particle Size: 10 µm, Pore Size: 100Å, Length: 250 mm, I. D.: 4.6 mm

SMT-WCX-10-100/25

€970.00

Product Type: HPLC Column Length: 250 mm Internal Diameter: 4.6 mm Particle Size: 10 µm Pore Size: 100 Å Chemistry: Weak cation-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 340.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

WCX-10-100/15 Ion-Exchange WCX Column Particle Size: 10 µm, Pore Size: 100Å, Length: 150 mm, I. D.: 4.6 mm

SMT-WCX-10-100/15

€937.00

Product Type: HPLC Column Length: 150 mm Internal Diameter: 4.6 mm Particle Size: 10 µm Pore Size: 100 Å Chemistry: Weak cation-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 340.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

WAX-5-300/25 Ion-Exchange WAX Column Particle Size: 5 µm, Pore Size: 300Å, Length: 250 mm, I. D.: 4.6 mm

SMT-WAX-5-300/25

€970.00

Product Type: HPLC Column Length: 250 mm Internal Diameter: 4.6 mm Particle Size: 5 µm Pore Size: 300 Å Chemistry: Weak anion-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 90.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

WAX-5-300/15 Ion-Exchange WAX Column Particle Size: 5 µm, Pore Size: 300Å, Length: 150 mm, I. D.: 4.6 mm

SMT-WAX-5-300/15

€937.00

Product Type: HPLC Column Length: 150 mm Internal Diameter: 4.6 mm Particle Size: 5 µm Pore Size: 300 Å Chemistry: Weak anion-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 90.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

WAX-5-100/25 Ion-Exchange WAX Column Particle Size: 5 µm, Pore Size: 100Å, Length: 250 mm, I. D.: 4.6 mm

SMT-WAX-5-100/25

€970.00

Product Type: HPLC Column Length: 250 mm Internal Diameter: 4.6 mm Particle Size: 5 µm Pore Size: 100 Å Chemistry: Weak anion-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 340.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

WAX-5-100/15 Ion-Exchange WAX Column Particle Size: 5 µm, Pore Size: 100Å, Length: 150 mm, I. D.: 4.6 mm

SMT-WAX-5-100/15

€937.00

Product Type: HPLC Column Length: 150 mm Internal Diameter: 4.6 mm Particle Size: 5 µm Pore Size: 100 Å Chemistry: Weak anion-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 340.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

WAX-10-300/25 Ion-Exchange WAX Column Particle Size: 10 µm, Pore Size: 300Å, Length: 250 mm, I. D.: 4.6 mm

SMT-WAX-10-300/25

€970.00

Product Type: HPLC Column Length: 250 mm Internal Diameter: 4.6 mm Particle Size: 10 µm Pore Size: 300 Å Chemistry: Weak anion-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 90.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

WAX-10-300/15 Ion-Exchange WAX Column Particle Size: 10 µm, Pore Size: 300Å, Length: 150 mm, I. D.: 4.6 mm

SMT-WAX-10-300/15

€937.00

Product Type: HPLC Column Length: 150 mm Internal Diameter: 4.6 mm Particle Size: 10 µm Pore Size: 300 Å Chemistry: Weak anion-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 90.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

WAX-10-100/25 Ion-Exchange WAX Column Particle Size: 10 µm, Pore Size: 100Å, Length: 250 mm, I. D.: 4.6 mm

SMT-WAX-10-100/25

€970.00

Product Type: HPLC Column Length: 250 mm Internal Diameter: 4.6 mm Particle Size: 10 µm Pore Size: 100 Å Chemistry: Weak anion-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 340.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

WAX-10-100/15 Ion-Exchange WAX Column Particle Size: 10 µm, Pore Size: 100Å, Length: 150 mm, I. D.: 4.6 mm

SMT-WAX-10-100/15

€937.00

Product Type: HPLC Column Length: 150 mm Internal Diameter: 4.6 mm Particle Size: 10 µm Pore Size: 100 Å Chemistry: Weak anion-exchange Manufacturer: SMT-Separation Methods Technologies Delivery Time: approx. 10 days Column Type: Analytical Column Bet Surface Area [m2/g]: 340.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous Technology: HPLC

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Technical Data

Reversal phase

Phase	Modification	Particle size	Pore size	Surface area	Carbon content	USP Code
SAM-C18 OD	C18	3, 5, 10 μm 5, 10 μm 5 μm	100 Å 300 Å 60 Å	340 m²/g 90 m²/g N/A	24 %C 8 %C N/A	L1
SAM-C18 ODL	C18	3, 5, 10 μm 5 μm	100 Å 300 Å	340 m²/g 90 m²/g	12 %C 4 %C	L1
SMT Elite-C18	C18	5, 10 μm	100 Å	340 m²/g	16 %C	L1
SAM-C8 O	C8	3, 5, 10 μm 5, 10 μm 5 μm	100 Å 300 Å 60 Å	340 m²/g 90 m²/g N/A	12 %C 5 %C N/A	L7
SAM-C8 OL	C8	3, 5, 10 μm 5, 10 μm	100 Å 300 Å	340 m²/g 90 m²/g	6 %C 2 %C	L7
SMT Elite-C8	C8	5, 10 μm	100 Å	340 m²/g	8 %C	L7
SMT MEB1	C1	5 μm 5 μm	100 Å 300 Å	340 m²/g 90 m²/g	1 %C 0.6 %C	L13
SMT MEB2	C2	5 μm 5 μm	100 Å 300 Å	340 m²/g 90 m²/g	2 %C 1 %C	L30
SMT MEB4	C4	5 μm 5 μm	100 Å 300 Å	340 m²/g 90 m²/g	4 %C 2 %C	L26
SMT Phen1	Ph (one per ligand)	5, 10 μm 5, 10 μm	100 Å 300 Å	340 m²/g 90 m²/g	7.1 %C 3.1 %C	L11
SMT Phen2	Ph (two per ligand)	5, 10 μm 5, 10 μm	100 Å 300 Å	340 m²/g 90 m²/g	8.4 %C 3.6 %C	L11
SMT PhenH	Ph-hexyl	3, 5, 10 μm 5, 10 μm	100 Å 300 Å	340 m²/g 90 m²/g	7.1 %C 3.1 %C	N/A
SMT SAM C3	C3	5 µm 5 µm	100 Å 300 Å	340 m²/g 90m²/g	6.5 %C 3.6 %C	N/A
SMT SAM C5 [Pen]	C5	5 µm 5 µm	100 Å 300 Å	340 m²/g 90 m²/g	6.5 %C 3.6 %C	N/A
SMT SAM C6 [Hex]	C6	5 µm 5 µm	100 Å 300 Å	340 m²/g 90 m²/g	6.5 %C 3.6 %C	N/A
SMT SAM C12 [DD]	C12	5 µm 5µm	100 Å 300 Å	340 m²/g 90 m²/g	11 %C 5 %C	N/A

Ion exchange

Phase	Modification	Particle size	Pore size	Surface area	USP Code
SMT SAX	quaternary amine	5, 10 μm 5, 10 μm	100 Å 300 Å	340 m²/g 90 m²/g	L14
SMT WAX	polyethyleneimine	5, 10 μm 5, 10 μm	100 Å 300 Å	340 m²/g 90 m²/g	N/A
SMT DEAE	di-ethyl-amino-ethyl	5, 10 μm 5, 10 μm	100 Å 300 Å	340 m²/g 90 m²/g	N/A
SMT SCX	sulfonic acid	5, 10 μm 5, 10 μm	100 Å 300 Å	340 m²/g 90 m²/g	L9, L52
SMT WCX	carboxylic acid	5, 10 μm 5, 10 μm	100 Å 300 Å	340 m²/g 90 m²/g	N/A

Affinity chromatography

Phase	Modification	Particle size	Pore size	Surface area	Carbon content	USP Code
SMT CIB-ProteinA	ProteinA	5, 10 μm	100 Å	N/A	N/A	N/A
SMT CIB-IgG	IgG	5, 10 µm	100 Å	N/A	N/A	N/A

Chiral phases

Phase

Modification

Particle size

Pore size

Surface area

Carbon content

USP Code

SMT ChiralSep1

N/A

5, 10 μm

5, 10 µm

100 Å

300 Å

N/A

SMT ChiralSep2

N/A

5, 10 µm

100 Å

300 Å

N/A

UHPLC phases

Phase	Modification	Particle size	Pore size	Surface area	Carbon content	USP Code
SMT SAM-C18	C18	1.5, 1.8 μm	100 Å	N/A	N/A	N/A
SMT SAM-C8	C8	1.5, 1.8 µm	100 Å	N/A	N/A	N/A

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