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Discovery
Discovery® is the brand name for various reversed-phase HPLC columns from the manufacturer Supelco™ (Sigma Aldrich™). The base material is a spherical, high-purity silica gel, which is available in various complementary modifications. Unique combinations of polar and hydrophobic retention mechanisms enable differentiated separations from octadecyl phases.
The Discovery® Zr series includes HPLC columns with zirconia-based stationary phases. The materials are not subject to shrinkage or swelling with changes in solvent, temperature, ionic strength, etc. and exhibit extremely high temperature and pH stability. The spherical metal oxide particles are available in four different reversed-phase modifications. Due to the additional occurrence of Lewis acid - Lewis base interactions as a retention mechanism, unique selectivities are achieved.
The Discovery® Bio series contains several wide-pore phases for the analysis of peptides and proteins.
From 01.09.2025 to 23.12.2025 you will receive a 20% discount on Discovery columns and guard columns.
Technical Data
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
| Discovery® HS C18 | C18 | 3, 5, 10 µm | 120 Å | + | 20% | L1 | 300 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® C18 | C18 | 5 µm | 180 Å | + | 12% | L1 | 200 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® C8 | C8 | 5 µm | 180 Å | + | 7.5% | L7 | 200 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® Cyano | Cyano | 5 µm | 180 Å | + | 4.5% | L10 | 200 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® HS F5 | Pentafluorophenylpropyl | 3, 5, 10 µm | 120 Å | + | 12% | L43 | 300 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® RP-AmideC16 | Palmitamidopropyl | 5 µm | 180 Å | + | 11% | N/A | 200 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® HS PEG | Polyetdylene glycol | 3, 5, 10 µm | 120 Å | N/A | N/A | N/A | 300 m2/g | N/A | N/A |
Discovery-Zr Series
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
| Discovery® Zr-PBD | Polybutadiene | 3, 5 µm | 300 Å | - | 2% | L49 | 30 m2/g | 1.0-13.0 | 100 °C[1] |
| Discovery® Zr-PS | Polystyrene | 3, 5 µm | 300 Å | - | 2% | N/A | 30 m2/g | 1.0-13.0 | 100 °C[1] |
| Discovery® Zr-Carbon C18 | C18-phenyl modified carbon | 3, 5 µm | 300 Å | - | 3% | N/A | 30 m2/g | 1.0-14.0 | 100 °C[2] |
| Discovery® Zr-Carbon | Graphitic like Carbon | 3, 5 µm | 300 Å | - | 1% | N/A | 30 m2/g | 1.0-14.0 | 100 °C[2] |
| Discovery® Zr-SAX | Polyetdyleneimine | 3 µm | 300 Å | - | 25% | N/A | 30 m2/g | 1.0-12.0 | 80 °C |
[1] Special hardware for operation between 100 °C and 150 °C is available
[2] Special hardware for operation between 100 °C and 200 °C is available
Discovery-Bio Series
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
| Discovery® BIO Wide Pore C18 | Polybutadiene | C18 | 3, 5, 10 µm | + | 9% | L1 | 100 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® BIO Wide Pore C8 | Polystyrene | C8 | 3, 5, 10 µm | + | 5% | L7 | 100 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® BIO Wide Pore C5 | C18-phenyl modified carbon | C8 | 33, 5, 10 µm | + | 3.5% | N/A | 100 m2/g | 2.0‒8.0 | 70 °C |
Downloads
Brochures
Merck Supelco Discovery BIO Brochure
Merck Supelco Discovery BIO Wide Pore HPLC Columns Catalogue 2019
Merck Supelco Discovery HS PEG Brochure
Applications
Merck Supelco Applications-Notebook Vol1 HPLC, UHPLC, LC-MS, SFC, SPE
Merck Supelco Discovery HS F5 Analysis of Acrylamide in Fried Potato Chips Applications
Merck Supelco Discovery HS F5 Analysis of Acrylamide and Acrylic Acid Applications
Merck Supelco Discovery Method Development Guide
Merck Supelco Discovery Zr Method Development Guide
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