Kromasil 100-10-C18(w) 20 x 250 mm

The Kromasil 100 C18(w) HPLC column 250x20mm, 10µm, is a wettable octadecyl phase (other designations: ODS, RP-18, C18) on 100 Å silica with polar embedded endcapping. The C18(w) surface has been developed for strongly aqueous conditions and can provide stable retention of polar compounds even with a 100% aqueous mobile phase. Kromasil 100 C18(w) is used in reversed-phase chromatography and offers an alternative selectivity to classic C18. Typical applications include organic acids, water-soluble active substances, polar metabolites, peptides, API analysis and samples analysed directly from aqueous matrices.

Performance Characteristics and Benefits

• Spherical, fully porous silica with high mechanical stability
• High surface area and high pore volume for efficient separations and good loading capacity
• Narrow particle size distribution for high efficiency and reproducible results
• Broad phase spectrum for reverse-phase, normal-phase, HILIC, and SFC
• Suitable for analytical method development, routine analysis, scale-up, and preparative applications

Additional Information

The Kromasil series is based on highly stable, spherical, and fully porous silica particles. The silica matrix combines a high surface area, high pore volume, and a controlled pore structure with a narrow particle size distribution. This supports efficient packing beds, low pressure drops relative to performance, and good column and batch reproducibility. The Kromasil platform offers different pore sizes: 60 Å for small organic molecules, 100 Å for small molecules and peptides, and 300 Å for proteins and larger biomolecules. Appropriate Kromasil pre-column cartridges can be used to protect the analytical packing bed from particles, sample matrix, and hard-to-elute components.

Legal Notices

Kromasil is a registered trademark of the manufacturer Nouryon, Bohus, Sweden.