Kromasil 300-16-C4 20 x 100 mm

The Kromasil 300 C4 HPLC column 100x20mm, 16µm, has a butyl modification on 300 Å wide-pore silica. The larger pore size makes the surface accessible to proteins and larger peptides, while the C4 chain provides moderate hydrophobic retention. Kromasil 300 C4 is used in reversed-phase chromatography and is especially suitable for biomolecules that are too strongly retained on C8 or C18 phases. Typical applications are protein analysis, peptide mapping, biopharmaceuticals, purity testing, BSA digests and methods for larger hydrophobic molecules.

Performance Characteristics and Benefits

• Spherical, fully porous silica with high mechanical stability
• High surface area and high pore volume for efficient separations and good loading capacity
• Narrow particle size distribution for high efficiency and reproducible results
• Broad phase spectrum for reverse-phase, normal-phase, HILIC, and SFC
• Suitable for analytical method development, routine analysis, scale-up, and preparative applications

Additional Information

The Kromasil series is based on highly stable, spherical, and fully porous silica particles. The silica matrix combines a high surface area, high pore volume, and a controlled pore structure with a narrow particle size distribution. This supports efficient packing beds, low pressure drops relative to performance, and good column and batch reproducibility. The Kromasil platform offers different pore sizes: 60 Å for small organic molecules, 100 Å for small molecules and peptides, and 300 Å for proteins and larger biomolecules. Appropriate Kromasil pre-column cartridges can be used to protect the analytical packing bed from particles, sample matrix, and hard-to-elute components.

Legal Notices

Kromasil is a registered trademark of the manufacturer Nouryon, Bohus, Sweden.