Kromasil 300-16-C18 20 x 50 mm

The Kromasil 300 C18 HPLC column 50x20mm, 16"length × ID" mm, "particle size" µm, is based on an octadecyl phase (other designations: ODS, RP-18, C18) on 300 Å wide-pore silica. The C18 modification generates strong hydrophobic interactions, while the larger pore structure remains accessible for proteins, peptides and larger biomolecules. Kromasil 300 C18 is used in reversed-phase chromatography when large molecules are to be separated with hydrophobic selectivity. Typical applications are peptides, proteins, biopharmaceutical active ingredients, peptide mapping, purity analysis and preparative separations of larger hydrophobic compounds.

Performance Characteristics and Benefits

• Spherical, fully porous silica with high mechanical stability
• High surface area and high pore volume for efficient separations and good loading capacity
• Narrow particle size distribution for high efficiency and reproducible results
• Broad phase spectrum for reverse-phase, normal-phase, HILIC, and SFC
• Suitable for analytical method development, routine analysis, scale-up, and preparative applications

Additional Information

The Kromasil series is based on highly stable, spherical, and fully porous silica particles. The silica matrix combines a high surface area, high pore volume, and a controlled pore structure with a narrow particle size distribution. This supports efficient packing beds, low pressure drops relative to performance, and good column and batch reproducibility. The Kromasil platform offers different pore sizes: 60 Å for small organic molecules, 100 Å for small molecules and peptides, and 300 Å for proteins and larger biomolecules. Appropriate Kromasil pre-column cartridges can be used to protect the analytical packing bed from particles, sample matrix, and hard-to-elute components.

Legal Notices

Kromasil is a registered trademark of the manufacturer Nouryon, Bohus, Sweden.