ACE 1.7 HILIC-A 100x2.1mm

The Avantor ACE HILIC-A HPLC/UHPLC column 100x2.1mm, 1.7µm, is based on a proprietary silica-based HILIC phase for hydrophilic interaction chromatography. The Avantor ACE HILIC-A exhibits an acidic character and can additionally form cation-exchange-like interactions with basic or positively charged analytes. This makes the phase suitable for polar substances that show little retention on reversed phases. Typical applications include beta blockers, creatinine, metabolic markers, neomycin, fluorodeoxyglucose, metformin and polar pharmaceutical active ingredients.

Features and Benefits

• Three proprietary HILIC phases for systematic method development
• Retention of polar and highly polar analytes outside the scope of conventional reverse-phase separation
• Distinct acidic, basic, and neutral selectivity profiles
• Ultra-inert silica base for reproducible HILIC separations
• Suitable for LC-MS-friendly mobile phases with a high organic content

Additional Notes

Avantor ACE HILIC uses ultra-inert silica particles with various proprietary polar surface chemistries. The three HILIC phases—HILIC-A, HILIC-B, and HILIC-N—were developed for systematic selectivity screening, as they represent different ionic and neutral interactions. In contrast to reverse-phase chromatography, retention in HILIC is typically determined by a water-rich layer on the polar stationary phase and mobile phases with a high organic content. Appropriate guard cartridges protect the sensitive HILIC method from matrix components and help ensure stable retention times.

Legal Notices

Avantor ACE is a registered trademark of the manufacturer Avantor, Inc., Radnor, USA.