ACE Excel OLIGO 5µM 250 X 4.6mm

The Avantor ACE Excel Oligo HPLC/UHPLC column 250x4.6mm, 5µm, features a proprietary encapsulated stationary phase for ion-pair reversed-phase chromatography of oligonucleotides. The Avantor ACE Excel Oligo is designed for high resolution, high pH stability and compatibility with typical LC-UV and LC-MS conditions for oligonucleotides. The phase can be used with mobile phases and buffer systems such as TEAA or HFIP while exhibiting low column bleed for sensitive MS detection. Typical applications are synthetic oligonucleotides, oligonucleotide ladders, therapeutic oligonucleotides, purity testing, sequence variants and method development for ion-pair based reversed-phase separations.

Features and Benefits

• Proprietary, encapsulated stationary phase for oligonucleotides
• Ion-pair reverse-phase chromatography compatible with LC-UV and LC-MS
• High pH stability for common oligonucleotide methods
• Ultra-inert, base-deactivated silica for reproducible peak shapes
• Low column bleed for sensitive MS detection

Additional Information

Avantor ACE Excel Oligo is based on an ultra-inert, base-deactivated silica backbone with proprietary stationary-phase encapsulation. This surface chemistry is designed to meet the high demands of oligonucleotide analysis, where pH stability, low column bleeding, and reproducible retention are critical. The material is designed for mobile phases and buffers used in ion-pair reverse-phase analysis of oligonucleotides. Appropriate guard cartridges protect the analytical oligo column from matrix interference without altering the selective chemistry of the main column.

Legal Notices

Avantor ACE is a registered trademark of the manufacturer Avantor, Inc., Radnor, USA.