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Mixed Mode Phases
Mixed-mode phases for HPLC utilise various separation techniques in parallel within a single column. The stationary phases are specially modified for this purpose, so that, for example, reversed-phase and ion-exchange properties contribute to the separation simultaneously. Nowadays, there are many different mixed-mode phases that combine a wide variety of separation modes. They exhibit unique selectivities, making them the column of choice for some difficult separations.
Technical Data
How do I choose the right mixed mode column?
The selection of the correct mixed mode column depends on the analytes and their ability to enter into certain interactions. For example, if a sample contains anionic and non-polar analytes, a mixed-mode column with anion exchange and reversed phase properties should be selected. Up to four different interaction mechanisms can take place simultaneously in one column, whereby the mobile phase is often decisive for the type of interaction or the strength of the interaction. As the selection depends on the analytes in a sample, it is difficult to establish general selection criteria. A literature search or an application search with the manufacturers should help you to choose the right column. We are happy to support you in selecting the right column. Please contact us!
Manufacturer of mixed-mode columns
| Manufacturer | Phase name | Modification | Separation mechanisms involved | Particle size | Pore size | pH range |
| Imtakt | Scherzo SS-C18 | C18 + SAX + SCX | Reversed phase Anion Exchange Cation Exchange Normal Phase | 3 µm | 130 Å | N/A |
Scherzo SM-C18 | C18 + WAX + WCX | Reversed phase Anion Exchange Cation Exchange Normal Phase | 3 µm | 130 Å | N/A | |
Scherzo SW-C18 | C18 + SAX + SCX | Reversed phase Anion Exchange Cation Exchange Normal Phase | 3 µm | 130 Å | N/A | |
| Shodex | Asahipak GS-HQ[1] | N/A | Size Exclusion (SEC) Reversed Phase HILIC | 6, 7 & 13 µm | 150, 400, | 2.0-9.0 |
| Sepax | HP-SCX | Sulfonic Acid + Phenyl | Cation Exchange Reversed Phase | 1.8, 3, 5 & 10 µm | 120 Å | 1.5-8.0 |
HP-SAX | Quat. Ammonium + Phenyl | Anion Exchange Reversed Phase | 5 µm | 120 Å | 1.5-8.0 | |
| Osaka Soda | Capcell Pak CR | C18 + SCX | Reversed Phase Cation Exchange | 3 & 5 µm | N/A | N/A |
| Thermo Scientific | Acclaim Mixed-Mode HILIC-1 | Alkyl chain with | Reversed-Phase HILIC | 3 & 5 µm | 120 Å | 2.5-7.5 |
Acclaim Mixed-Mode WAX-1 | Alkyl chain with | Reversed Phase Anion Exchange | 3 & 5 µm | 120 Å | 2.5-7.5 | |
Acclaim Mixed-Mode WCX-1 | Alkyl chain with | Reversed Phase Cation Exchange Anion Exclusion | 3 & 5 µm | 120 Å | 2.5-7.5 | |
Acclaim Trinity P1 | RP + SCX + WAX | Reversed Phase Anion Exchange Cation Exchange | 3 µm | 300 Å | 2.5-7.0 | |
Acclaim Trinity P2 | WCX + SAX | HILIC Anion Exchange Cation Exchange | 3 µm | 300 Å | 2.0-8.0 | |
Acclaim Trinity Q1 | RP + WCX + WAX | Reversed Phase Anion Exchange Cation Exchange | 3 µm | 300 Å | 2.5-7.5 | |
| Sielc | Primesep A | RP + embedded SCX | Reversed Phase Cation Exchange | 5 & 10 µm | 100 Å | N/A |
Primesep 100 | RP + embedded CX | Reversed Phase Cation Exchange | 2.7, 3, 5, & 10 µm | 100 Å | N/A | |
Primesep 200 | RP + embedded WCX | Reversed Phase Cation Exchange | 5 & 10 µm | 100 Å | N/A | |
Primesep B | RP + embedded SAX | Reversed Phase Anion Exchange | 5 & 10 µm | 100 Å | N/A | |
Primesep B2 | RP + embedded WAX | Reversed Phase Anion Exchange | 5 & 10 µm | 100 Å | N/A | |
Primesep AB | RP + embedded AX + CX | Reversed-Phase Anion Exchange Cation Exchange | 5 & 10 µm | 100 Å | N/A | |
Primesep C | Proprietary | Reversed Phase Cation Exchange Complex Formation | 5 & 10 µm | 100 Å | N/A |
[1] Base Material: Polyvinylalcohol
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