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Glyphosat
Glyphosate - Analysis of a Controversial Plant Protection Product
Glyphosate is an herbicide that has been on the market since the 1970s and has since become the most commonly used weedkiller. The use of glyphosate is highly controversial among experts, users and consumers, as it is suspected of being carcinogenic.
Various liquid chromatography separation techniques can be used to analyse glyphosate and its metabolites (e.g. aminomethylphosphoric acid - AMPA). These include ion chromatography, hydrophilic interaction chromatography (HILIC) and reversed-phase chromatography. Due to the high polarity of glyphosate and its UV inactivity, the amino nitrogen is often derivatised with an Fmoc protecting group during sample preparation, which facilitates the separation and detection of the resulting substances.
Applications
HILIC LC/MS analysis of glyphosate and glufosinate with Shodex HILICpak VT-50 2D
Peak identities
1. AMPA 2. glufosinates 3. glyphosates 4. MPPA (3-methylphosphinicopropionic acid)
Test conditions
Column: HILICpak VT-50 2D 150x2.0mm, 5µm
Mobile phase: 70/20/10 water/1% formic acid/acetonitrile
Flow rate: 0.3 mL/min
Detection: ESI-MS (SIM)
Temperature: 40 °C
Sample: 1 µg/mL
Injection volume: 5 µL
Ion chromatography of glyphosate and glufosinate with Thermo IC SI-90 4E
Peak identities
1. glufosinates 2. glyphosates
Test conditions
Column: IC SI-90 4E 250x4.0mm, 9µm
Mobile phase A: 0.3 mM NaOH
Mobile phase B: 0.3 mM NaOH + 20 mM sodium carbonate
Gradient:
Time / min | %A | %B |
0 | 95 | 5 |
20 | 95 | 5 |
30 | 50 | 50 |
40 | 50 | 50 |
Flow rate: 1 mL/min
Suppressor: Dionex AMMS-ICS II
Detector: ICP/MS, P at m/Z=31
Reversed Phase Analysis of Glyphosate with GL SciencesInertsil ODS-4
Peak identities
1. glyphosates
Test conditions
Column: Inertsil ODS-4 150x4.6mm, 5µm (5020-03945)
Mobile phase A: Acetonitrile
Mobile phase B: 50 mM potassium dihydrogen phosphate (pH 2.5 with phosphoric acid)
Gradient: 35%A - 2.5 min, 70%A - 3 min, 35%A - 15.5 min
Flow rate: 1 mL/min
Temperature: 40 °C
Detection: Fluorescence excitation 270 nm, emission 315 nm
Injection volume: 10 µL
Downloads
Manufacturer overview
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Avantor ACE
HPLC - Reversed Phase:
- Avantor ACE Excel 3 Super C18, 3µm
Concise
HPLC - IEX:
- Glyphosate Analysis Column
GL Sciences
HPLC - Reversed Phase:
- Inertsil ODS-4, 5µm
- InertSustainSwift C18, 3µm
Imtakt
HPLC - Mixed-Mode:
- Scherzo SM-C18, 3µm
Macherey-Nagel
HPLC - Reversed Phase:
- Nucleoshell RP-18, 2.7µm
MicroSolv
HPLC - HILIC:
- Cogent Diamond Hydride, 4µm
Shodex
HPLC - HILIC:
- HILICpak VT-50 2D
HPLC - Ion chromatography:
- IC SI-90 4E
Thermo Fisher
HPLC - Ion chromatography:
- Dionex IonPac AS24, 7µm
HPLC - Reversed Phase
- Hypercarb, 5µm
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