ACE 3 C4-300 100 X0.30mm 1/32 FITT

The Avantor ACE C4-300 HPLC column 100x0.3mm, 3µm, is a wide-pore butyl phase with 300 Å pores for reversed-phase chromatography of biomolecules. The Avantor ACE C4-300 exhibits lower hydrophobicity than C8-300 or C18-300 and is suitable for proteins and peptides that should be eluted under milder reversed-phase conditions. The large pore structure improves access of larger molecules to the stationary phase. Typical applications include preparative fractionations, alpha-lactalbumin, peptide mixtures, proteins and biological therapeutics with pronounced hydrophobic sensitivity.

Performance Characteristics and Benefits

• Ultra-inert, high-purity, base-deactivated silica particles
• Broad range of selectivity for reverse-phase, normal-phase, and HILIC applications
• Fully porous 100 Å and 300 Å phases for small molecules and biomolecules
• Excellent peak shapes for acidic, basic, and neutral analytes
• High reproducibility from column to column and batch to batch

Additional Information

The Avantor ACE Series is based on high-purity, spherical silica with very low metal contamination and targeted base deactivation. Controlled surface chemistry reduces the activity of free silanol groups, which promotes symmetrical peaks and reproducible retention times, particularly for basic analytes. The portfolio includes classic fully porous 100 Å phases, alternative selectivities, and 300 Å wide-pore materials for larger molecules. Using compatible Avantor ACE guard cartridges protects the silica backbone of the analytical column from matrix components and can extend the column’s service life.

Legal Notices

Avantor ACE is a registered trademark of the manufacturer Avantor, Inc., Radnor, USA.