ACE 2 Silica Microbore HPLC Column 75x0.5mm

The Avantor ACE SIL HPLC column 75x0.5mm, 2µm, is made of unmodified, high-purity silica and is used primarily in normal phase chromatography. The Avantor ACE SIL exploits polar silanol interactions and is suitable for analytes that are not sufficiently retained on reversed phases. The phase offers a classical silica selectivity with good reproducibility and can be used for sensitive methods with non-polar mobile phases. Typical applications are lipids, fat-soluble vitamins, tocopherols, isomers, natural products and separations where adsorption chromatography is predominant.

Performance Characteristics and Benefits

• Ultra-inert, high-purity, base-deactivated silica particles
• Broad range of selectivity for reverse-phase, normal-phase, and HILIC applications
• Fully porous 100 Å and 300 Å phases for small molecules and biomolecules
• Excellent peak shapes for acidic, basic, and neutral analytes
• High reproducibility from column to column and batch to batch

Additional Information

The Avantor ACE Series is based on high-purity, spherical silica with very low metal contamination and targeted base deactivation. Controlled surface chemistry reduces the activity of free silanol groups, which promotes symmetrical peaks and reproducible retention times, particularly for basic analytes. The portfolio includes classic fully porous 100 Å phases, alternative selectivities, and 300 Å wide-pore materials for larger molecules. Using compatible Avantor ACE guard cartridges protects the silica backbone of the analytical column from matrix components and can extend the column’s service life.

Legal Notices

Avantor ACE is a registered trademark of the manufacturer Avantor, Inc., Radnor, USA.