Ascentis® C18 HPLC Column, 5 µm particle size, L x I.D. 25 cm x 10 mm

The Ascentis C18 HPLC column 250x10mm, 5µm, is based on an octadecyl phase (also known as ODS, RP-18, C18) for reversed phase chromatography. The high-purity Type B silica of the Ascentis C18 is endcapped and provides robust retention for neutral, acidic and basic compounds. As a universal C18 column the Ascentis C18 is frequently used in pharmaceutical methods, environmental analysis, food analysis and routine testing.

Features and Benefits

• High-purity Type B silica
• Wide selection of RP, NP, and specialty phases
• Good peak shapes for acidic, neutral, and basic analytes
• Analytical and preparative formats available
• Compatible Supelguard column protection systems

Additional Notes

The Ascentis series is based on spherical, high-purity silica with a controlled pore structure. Depending on the modification, hydrophobic, polar, aromatic, or normal-phase interactions are emphasized. As a result, Ascentis can be used for both classical reverse-phase methods and more selective method developments. The use of pre-column cartridges or guard columns in the same phase protects the analytical column from particles and highly retained matrix components without significantly altering the selectivity of the method.

Legal Information

Ascentis is a registered trademark of the manufacturer Merck KGaA, Darmstadt, Germany.