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The Discovery C18 HPLC column 250x21.2mm, 5µm, utilises a C18 modification with a larger pore structure than many traditional standard phases. The Discovery C18 is used in reversed-phase chromatography when stronger retention of hydrophobic analytes and an established alkyl phase selectivity are required. Applications of the Discovery C18 include pharmaceutical analysis, natural product analysis, food control and environmental samples.
The Discovery Series includes various material platforms and surface chemistries. In addition to classic silica-based reverse-phase columns, high-purity HS phases, pentafluorophenyl, RP-amide, and PEG selectivities, as well as zirconia-based phases, are available. Bio-Wide-Pore variants with larger pores are designed for peptides and proteins. This makes Discovery particularly well-suited for method development when standard C18 selectivity is insufficient or more robust surfaces are required.
Discovery is a registered trademark of the manufacturer Merck KGaA, Darmstadt, Germany.
| Brand | Discovery |
|---|---|
| Product Type | HPLC Column |
| Length | 250 mm |
| Internal Diameter | 21.2 mm |
| Particle Size | 5 µm |
| Chemistry | C18 (RP-18, ODS, Octadecyl) |
| Chemistry Producers Term | C18 |
| Pore Size | 180 Å |
| USP Class | L1 |
| Carbon Load | 12.0 % |
| Bet Surface Area [m2/g] | 200.0 |
| Endcapping | endcapped |
| Country of Origin | United States of America (USA) |
| Column Type | Preparative Column |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Temperature Maximum [°C] | 70 |
| Mode of Separation | Reversed Phase Chromatography (RP) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |
| Pressure Stability | 400 bar |