Discovery® BIO Wide Pore C18 HPLC Column, 10 µm particle size, L x I.D. 25 cm x 10 mm

Part Number: SU-567209-U
Manufacturer: Merck Supelco
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Delivery Time: approx. 3 days
Excl. 19% taxes, excl. Shipping costs
Packaging unit: 1 pc, Minimum order quantity: 1 pc
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Product Description

The Discovery BIO Wide Pore C18 HPLC column 250x10mm, 10µm, is a C18 phase with a larger pore structure for biomolecules. The Discovery BIO Wide Pore C18 facilitates access of larger peptides and proteins to the surface and is used in RP bio-LC methods. Typical applications of the Discovery BIO Wide Pore C18 are peptide mapping, protein fragments, biopharmaceutical samples and LC-MS analysis.

Features and Benefits

  • Wide range of selectivities
  • Silica, zirconia, and Bio-Wide-Pore phases
  • Suitable for RP, specialized RP, and bioanalytics
  • Robust method development
  • Compatible Supelguard column protection systems

Additional Information

The Discovery Series includes various material platforms and surface chemistries. In addition to classic silica-based reverse-phase columns, high-purity HS phases, pentafluorophenyl, RP-amide, and PEG selectivities, as well as zirconia-based phases, are available. Bio-Wide-Pore variants with larger pores are designed for peptides and proteins. This makes Discovery particularly well-suited for method development when standard C18 selectivity is insufficient or more robust surfaces are required.

Legal Information

Discovery is a registered trademark of the manufacturer Merck KGaA, Darmstadt, Germany.

Product Specification

Brand Discovery Bio
Product Type HPLC Column
Length 250 mm
Internal Diameter 10 mm
Particle Size 10 µm
Chemistry C18 (RP-18, ODS, Octadecyl)
Chemistry Producers Term C18
Pore Size 300 Å
USP Class L1
Carbon Load 9.0 %
Bet Surface Area [m2/g] 100.0
Endcapping endcapped
Country of Origin United States of America (USA)
Column Type Semi-preparative Column
Hardware Material Stainless Steel
Hardware Type Ready-to-use Column
pH Minimum 2.0
pH Maximum 8.0
Temperature Maximum [°C] 70
Mode of Separation Reversed Phase Chromatography (RP)
Technology HPLC
Matrix Silica
Particle Morphology spherical, fully porous

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