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The Ascentis Express Peptide ES-C18 HPLC-/UHPLC column 50x0.2mm, 2.7µm, is designed as a C18 phase with an enlarged pore structure for peptide separations. The Ascentis Express Peptide ES-C18 enables good mass transport for larger molecules and is preferred in LC-MS methods, proteomics workflows, peptide mapping and bioanalysis. Due to the non endcapped surface, peptides can be separated under acidic conditions with differentiated selectivity.
Ascentis Express columns are based on fused-core or superficially porous particles. A solid core combined with a porous shell provides short diffusion paths and an efficient packing structure. As a result, high column numbers and narrow peaks can be achieved, often without reaching the backpressure associated with fully porous sub-2-µm particles. The platform includes classic reverse-phase columns, polar embedded phases, HILIC phases, and application-specific surface chemistries.
Ascentis Express is a registered trademark of the manufacturer Merck KGaA, Darmstadt, Germany.
| Brand | Ascentis Express |
|---|---|
| Product Type | CoreShell Column |
| Length | 50 mm |
| Internal Diameter | 0.2 mm |
| Particle Size | 2.7 µm |
| Chemistry | C18 (RP-18, ODS, Octadecyl) |
| Chemistry Producers Term | Peptide ES-C18 |
| Pore Size | 160 Å |
| USP Class | L1 |
| Carbon Load | 4.6 % |
| Bet Surface Area [m2/g] | 90.0 |
| Endcapping | non endcapped |
| Country of Origin | Germany |
| Column Type | Capillary Column |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 1.0 |
| pH Maximum | 9.0 |
| Mode of Separation | Reversed Phase Chromatography (RP) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, solid core, porous shell |