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The Ascentis Express OH5 HPLC-/UHPLC column 150x2.1mm, 5µm, uses a penta‑hydroxylated surface for hydrophilic interaction chromatography.The Ascentis Express OH5 is polar‑neutral in design and is suitable for HILIC separations where hydrogen bonding and hydrophilic interactions predominate.Typical applications are sugars, glycans, nucleotides, polar metabolites and very hydrophilic small molecules.
Ascentis Express columns are based on fused-core or superficially porous particles. A solid core combined with a porous shell provides short diffusion paths and an efficient packing structure. As a result, high column numbers and narrow peaks can be achieved, often without reaching the backpressure associated with fully porous sub-2-µm particles. The platform includes classic reverse-phase columns, polar embedded phases, HILIC phases, and application-specific surface chemistries.
Ascentis Express is a registered trademark of the manufacturer Merck KGaA, Darmstadt, Germany.
| Brand | Ascentis Express |
|---|---|
| Product Type | CoreShell Column |
| Length | 150 mm |
| Internal Diameter | 2.1 mm |
| Particle Size | 5 µm |
| Chemistry | HILIC |
| Chemistry Producers Term | OH5 |
| Pore Size | 90 Å |
| Carbon Load | 2.1 % |
| Bet Surface Area [m2/g] | 90.0 |
| Endcapping | endcapped |
| Country of Origin | Germany |
| Column Type | Analytical Column |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 1.0 |
| pH Maximum | 8.0 |
| Mode of Separation | Hydrophilic Interaction Chromatography (HILIC) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, solid core, porous shell |