Inertsil WP300-C8 5µm column 3.9 x 150mm HPLC Column

The Inertsil WP300-C8 HPLC column 150x3.9mm, 5µm, is a wide-pore octyl phase (also known as RP-8, C8) with a pore size of 300 Å for reverse-phase chromatography. Inertsil WP300 C8 combines the milder hydrophobic retention of a C8 phase with a pore structure that is accessible to larger molecules. This makes the column particularly suitable for proteins, peptides, and other biomolecules, where standard pores can lead to limited interaction. Typical applications include bioanalytics, protein and peptide separations, purity analysis, and biopharmaceutical methods.

Performance Characteristics and Benefits

• High-purity silica
• Controlled pore structure and reproducible surface
• High inertness due to reduced silanol and metal activity
• Excellent peak shapes for acidic, neutral, and basic analytes
• Reliable reproducibility for routine analysis

Additional Notes

The Inertsil series is based on a high-purity, spherical silica matrix whose pore structure, particle size distribution, and surface are carefully controlled during production. Low metal contamination and careful surface treatment reduce the activity of free silanol groups. This minimizes undesirable secondary interactions—particularly tailing and adsorption with basic or chelating analytes—and promotes stable retention times. This reproducible silica matrix forms the basis for the various Inertsil modifications and ensures reliable selectivities across the series. Appropriate Inertsil pre-column cartridges should be used to protect the analytical packing bed from particles and the sample matrix and to extend column lifespan.

Legal Notices

Inertsil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.