Inertsil WP300-C4 5µm CapillaryEx-Nano column 0.2 x 250mm HPLC Column

The Inertsil WP300 C4 HPLC column 250x0.2mm, 5µm, is based on a butyl phase on wide-pore silica with a pore size of 300 Å. Inertsil WP300 C4 is designed for large, highly hydrophobic proteins and peptides, and its shorter alkyl chain reduces retention compared to C8 and C18 phases. In addition, the optimized surface chemistry supports good peak shapes for basic and fat-soluble biomolecules. Typical applications include protein analysis, peptide analysis, biopharmaceutical QC, and methods involving large hydrophobic biomolecules.

Performance Characteristics and Benefits

• High-purity silica
• Controlled pore structure and reproducible surface
• High inertness due to reduced silanol and metal activity
• Excellent peak shapes for acidic, neutral, and basic analytes
• Reliable reproducibility for routine analysis

Additional Notes

The Inertsil series is based on a high-purity, spherical silica matrix whose pore structure, particle size distribution, and surface are carefully controlled during production. Low metal contamination and careful surface treatment reduce the activity of free silanol groups. This minimizes undesirable secondary interactions—particularly tailing and adsorption with basic or chelating analytes—and promotes stable retention times. This reproducible silica matrix forms the basis for the various Inertsil modifications and ensures reliable selectivities across the series. Appropriate Inertsil pre-column cartridges should be used to protect the analytical packing bed from particles and the sample matrix and to extend column lifespan.

Legal Notices

Inertsil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.