Inertsil PEPTIDES 4µm CapillaryEx-Nano column 0.2 x 250mm HPLC Column

The Inertsil Peptides C18 HPLC column 250x0.2mm, 4µm, uses an octadecyl modification (also known as ODS, RP-18, C18) on high-purity silica for reverse-phase analysis of peptides. Inertsil Peptides C18 is designed for peptide mapping, protein digestion analysis, and the separation of small to medium-sized peptide fragments. The 100-Å pore structure and high surface area support good resolution in peptide mixtures, while the end-capped packing material enables robust peak shapes. Typical applications include tryptic digests, peptide purity analysis, method development in proteomics, pharmaceutical and biopharmaceutical analysis, and QC methods for peptide active ingredients or synthetic peptides.

Performance Characteristics and Benefits

• High-purity silica
• Controlled pore structure and reproducible surface
• High inertness due to reduced silanol and metal activity
• Excellent peak shapes for acidic, neutral, and basic analytes
• Reliable reproducibility for routine analysis

Additional Notes

The Inertsil series is based on a high-purity, spherical silica matrix whose pore structure, particle size distribution, and surface are carefully controlled during production. Low metal contamination and careful surface treatment reduce the activity of free silanol groups. This minimizes undesirable secondary interactions—particularly tailing and adsorption with basic or chelating analytes—and promotes stable retention times. This reproducible silica matrix forms the basis for the various Inertsil modifications and ensures reliable selectivities across the series. Appropriate Inertsil pre-column cartridges should be used to protect the analytical packing bed from particles and the sample matrix and to extend column lifespan.

Legal Notices

Inertsil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.