ToyoScreen AF-Chelate-650M, 65 µm, 1.000 Å 5ml * 6 columns

ToyoScreen AF-Chelate-650M transfers TOYOPEARL AF-Chelate-650M with chelating groups for metal ion loading into a ready-to-use small format. The intended separation mode is immobilised metal affinity chromatography (IMAC). Different metal ions and buffer conditions can be systematically compared in the small format. The ToyoScreen AF-Chelate-650M supports automated or manual screening campaigns for His-tagged proteins, metalloproteins and IMAC method development.

Features and Benefits

• Pre-packaged screening formats
• Manual and automated experiments
• 200 µL to 5 mL bed volume
• TOYOPEARL polymers
• Direct scale-up to process formats

Additional Information

ToyoScreen offers TOYOPEARL ion-exchange, mixed-mode, HIC, and affinity media in reproducibly pre-packed small formats. The classic 1- and 5-mL columns are suitable for manual or automated method development; RoboColumns enable parallel experiments in liquid-handling systems. This allows for the systematic investigation of binding, washing conditions, gradients, elution, and regeneration using identical resin chemistry. Since the same TOYOPEARL media are subsequently available as bulk process resin or in SkillPak columns, ToyoScreen results support scale-up from design space screening to the process column.

Legal Information

ToyoScreen is a registered trademark of Tosoh Corporation, Tokyo, Japan.