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The Waters µBondapak C18 HPLC column 300x30mm, 15-20µm, is based on an octadecylsilyl phase (other designations: ODS, RP-18, C18) on irregular silica with classic µBondapak selectivity and is designed for reversed-phase chromatography. The historical C18 packing provides a broadly referenced selectivity and is important for older, validated HPLC methods. In practice the Waters µBondapak C18 is suitable, for example, for classical HPLC methods, preparative starting methods, active substance analysis and methods that explicitly require µBondapak chemistry.
µBondapak is a classic Waters HPLC platform based on fully porous 125-Å silica with 10-µm particles. The product family includes bonded C18, cyano, phenyl, and amino phases, covering reverse-phase, normal-phase, and polar selectivities. Due to its long history on the market, the original µBondapak selectivity is well-established in numerous pharmacopoeia, literature, and in-house methods. When transferring methods, selections should therefore be based not only on USP class but also on particle type, bonding chemistry, and historical column format.
µBondapak is a registered trademark of Waters Corporation, Milford, Massachusetts, USA.
| Brand | µBondapak |
|---|---|
| Product Type | HPLC Column |
| Length | 300 mm |
| Internal Diameter | 30 mm |
| Particle Size | 15-20 µm |
| Chemistry | C18 (RP-18, ODS, Octadecyl) |
| Chemistry Producers Term | C18 |
| Pore Size | 125 Å |
| USP Class | L1 |
| Carbon Load | 10.0 % |
| Bet Surface Area [m2/g] | 330.0 |
| Endcapping | endcapped |
| Country of Origin | United States of America (USA) |
| Column Type | Preparative Column |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Temperature Maximum [°C] | 45 |
| Mode of Separation | Reversed Phase Chromatography (RP) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | irregular, fully porous |
| Pressure Stability | 415 bar |