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The ACQUITY UPLC Protein BEH SEC 200 Å column 150x4.6mm, 1.7µm, is a diol-modified BEH-SEC phase for the analysis of proteins and protein aggregates. With a pore size of 200 Å, this modification typically covers a molecular weight range of approximately 10,000 to 450,000 Dalton and is therefore frequently used for monoclonal antibodies, IgG monomers, dimers and smaller aggregates. The ACQUITY UPLC Protein BEH SEC 200 Å combines the efficiency of a UPLC-compatible SEC column with a surface tuned for low adsorption and reproducible protein separations.
The Waters ACQUITY series includes sub-2-µm UPLC phases based on hybrid particles, high-strength silica, and charged-surface hybrid materials. BEH particles consist of an organic-inorganic hybrid matrix and offer high chemical and mechanical stability. HSS particles provide silica-like selectivity with high pressure stability, while CSH particles enable particularly good peak shapes for basic analytes in LC-MS-friendly mobile phases thanks to a controlled surface charge. Compatible VanGuard or Guard systems protect the main column from matrix components.
ACQUITY is a registered trademark of Waters Corporation, Milford, Massachusetts, USA.
| Brand | ACQUITY Protein |
|---|---|
| Product Type | SEC / GPC Columns |
| Length | 150 mm |
| Internal Diameter | 4.6 mm |
| Particle Size | 1.7 µm |
| Chemistry Producers Term | BEH SEC |
| Pore Size | 200 Å |
| USP Class | L33 |
| Carbon Load | 12.0 % |
| Bet Surface Area [m2/g] | 220.0 |
| Endcapping | non endcapped |
| Country of Origin | United States of America (USA) |
| Column Type | Analytical Column |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 1.0 |
| pH Maximum | 8.0 |
| Mode of Separation | SEC / Aqueous Size Exclusion Chromatography (GFC) |
| Technology | U(H)PLC |
| Matrix | Hybrid |
| Particle Morphology | spherical, fully porous |