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The Acclaim PepMap 100 C18 HPLC column 20x0.2mm, 5µm, based on highly pure, fully porous 100Å silica with an endcapped octadecyl modification (other designations: ODS, RP-18, C18). The reversed phase combines a high specific surface area and loading capacity with pronounced peptide retention and supports TFA-free LC-MS procedures.Nano-, capillary and micro formats as well as suitable trap columns and trap cartridges enable desalting, preconcentration and subsequent high-resolution separation.Acclaim PepMap 100 C18 is used in particular for tryptic, natural and synthetic peptides, peptide mapping, protein identification and LC-MS/MS-based proteomics.
The Acclaim product family includes classic reverse-phase, polar embedded, and HILIC phases, multimodal materials, and application-optimized columns for ions, surfactants, explosives, carbonyls, carbamates, and organic acids. The selection depends on the analyte’s polarity, charge state, desired retention mechanism, and detection method. For mixed-mode columns, the pH value, buffer type, ionic strength, and organic content must be optimized together; SEC ranges are always calibration-dependent reference values.
Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. Acclaim refers to a product line within the Thermo Scientific chromatography portfolio.
| Brand | Acclaim |
|---|---|
| Product Type | HPLC Column |
| Length | 20 mm |
| Internal Diameter | 0.2 mm |
| Particle Size | 5 µm |
| Country of Origin | Lithuania |
| Column Type | Capillary Column |
| Hardware Type | Ready-to-use Column |
| Mode of Separation | Reversed Phase Chromatography (RP) |
| Technology | HPLC |