Inertsil AX 5µm column 4.6 x 33mm HPLC Column

The Inertsil AX HPLC column 33x4.6mm, 5µm, is an anion-exchange phase with diethylamino groups bound to silica via an alkyl chain. Inertsil AX is used in ion-exchange chromatography and is particularly suitable for acidic compounds whose retention is controlled by ionic interactions. Retention is influenced, among other factors, by the type of buffer, buffer concentration, and pH. Typical applications include organic acids, acidic active ingredients, counterions, nucleotides, and polar ionic analytes.

Performance Characteristics and Benefits

• High-purity silica
• Controlled pore structure and reproducible surface
• High inertness due to reduced silanol and metal activity
• Excellent peak shapes for acidic, neutral, and basic analytes
• Reliable reproducibility for routine analysis

Additional Notes

The Inertsil series is based on a high-purity, spherical silica matrix whose pore structure, particle size distribution, and surface are carefully controlled during production. Low metal contamination and careful surface treatment reduce the activity of free silanol groups. This minimizes undesirable secondary interactions—particularly tailing and adsorption with basic or chelating analytes—and promotes stable retention times. This reproducible silica matrix forms the basis for the various Inertsil modifications and ensures reliable selectivities across the series. Appropriate Inertsil pre-column cartridges should be used to protect the analytical packing bed from particles and the sample matrix and to extend column lifespan.

Legal Notices

Inertsil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.