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PerfectSil 300 Diol 5µm 250x10mm HPLC Column
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Product Description
The PerfectSil 300 Diol column 250x10mm, 5µm, is a diol column that can be used in both reversed phase and normal-phase chromatography. However, it is more commonly used in normal-phase chromatography. PerfectSil 300 Diol can be used as a replacement for PerfectSil 300 Sil. The selectivity is comparable, but the PerfectSil 300 Diol has shorter equilibration times. The large pores of 300Å allow the PerfectSil 300 Diol to be used for the analysis of large compounds such as proteins, large peptides, and large natural products.
Features and benefits
- 300Å pores specifically for biomolecules
- High-purity silica gel
- High reproducibility
- Suitable for LC/MS
- Compatible with conventional HPLC systems
Further information
The PerfectSil 300 series was specially developed for the separation of biomolecules. The 300Å pores allow proteins to penetrate the pore system. This enables small peptides through to complex proteins to be separated and retained on the PerfectSil 300 columns. The high-purity silica gel and stable modification also allow use in LC/MS. The service life can be increased by using suitable pre-column cartridges.
Legal information
PerfectSil 300 is a registered trademark of the manufacturer MZ-Analysentechnik GmbH, Mainz, Germany.
Product Specification
| Brand | PerfectSil |
|---|---|
| Product Type | HPLC Column |
| Length | 250 mm |
| Internal Diameter | 10 mm |
| Particle Size | 5 µm |
| Chemistry | Diol |
| Chemistry Producers Term | Diol |
| Pore Size | 300 Å |
| USP Class | L20 |
| Carbon Load | 5.0 % |
| Bet Surface Area [m2/g] | 100.0 |
| Endcapping | non endcapped |
| Country of Origin | Germany |
| Column Type | Semi-preparative Column |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Temperature Maximum [°C] | 60 |
| Mode of Separation | Semipolar Phase Chromatography |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |
| Pressure Stability | 300 bar |
