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The PerfectChrom 100 C4 HPLC column 150x10mm, 5µm, is a C4 phase for reverse-phase chromatography. The PerfectChrom 100 C4 is often used when retention times on a C18 or C8 column are too long or when the analyte does not elute at all. In addition to the lower hydrophobic interaction, the silica matrix with polar interactions also plays a role, which can result in different selectivity compared to C18 or C8. Due to the low hydrophobicity of the PerfectChrom 100 C4, highly nonpolar compounds such as steroids, lipophilic metabolites, or small peptides can be analyzed.
The PerfectChrom series is based on a Type A silica gel. This means that some transition metals are present in the silica framework. As a result, the silanol groups are more acidic, which can lead to different interactions compared to modern Type B silica gels. The service life can be extended by using appropriate pre-column cartridges.
PerfectChrom is a registered trademark of the manufacturer MZ-Analysentechnik GmbH, Mainz, Germany.
| Brand | PerfectChrom |
|---|---|
| Product Type | HPLC Column |
| Length | 150 mm |
| Internal Diameter | 10 mm |
| Particle Size | 5 µm |
| Chemistry | C4 |
| Chemistry Producers Term | C4 |
| Pore Size | 100 Å |
| USP Class | L26 |
| Carbon Load | 6.0 % |
| Bet Surface Area [m2/g] | 350.0 |
| Endcapping | endcapped |
| Country of Origin | Germany |
| Column Type | Semi-preparative Column |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Temperature Maximum [°C] | 60 |
| Mode of Separation | Reversed Phase Chromatography (RP) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |
| Pressure Stability | 400 bar |