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SRT-C, Zenix-C, Unix-C
SRT-C, Zenix-C and Unix-C columns are silica based columns. Like the SRT, Zenix and Unix phases, the SRT-C, Zenix-C and Unix-C particles are coated with an uniform, hydrophilic and neutral film. However, this monolayer is a lay-down layer instead of a stand-up monolayer.
This is ideal for the separation of all biological samples types including challenging hydrophobic samples like antibody drug conjugates (ADCs), hydrophobic mAb and its fragments, bispecific mAbs (BsMAbs), PEGylated proteins, glycoproteins, fusion proteins, membrane proteins and oligonucleotides.
Technical Data
SRT-C
| Phase | Particle size | Pore size | Molecular range |
| SRT-C SEC-100 | 5, 10 μm | 100 Å | 100 - 100,000 (100 - 30,000 for 10 μm particle) Da |
| SRT-C SEC-150 | 5 μm | 150 Å | 500 - 150,000 Da |
| SRT-C SEC-300 | 5, 10 μm | 300 Å | 5,000 - 1,250,000 Da |
| SRT-C SEC-500 | 5, 10 μm | 500 Å | 15,000 - 5,000,000 Da |
| SRT-C SEC-1000 | 5, 10 μm | 1000 Å | 50,000 - 7,500,000 Da |
| SRT-C SEC-2000 | 5 μm | 2000 Å | >10,000,000 Da |
Zenix-C
| Phase | Particle size | Pore size | Molecular range |
| Zenix-C SEC-80 | 3 μm | 80 Å | 100 - 50,000 Da |
| Zenix-C SEC-100 | 3 μm | 100 Å | 100 - 100,000 Da |
| Zenix-C SEC-150 | 3 μm | 150 Å | 500 - 150,000 Da |
| Zenix-C SEC-300 | 3 μm | 300 Å | 5,000 - 1,250,000 Da |
Unix-C
| Phase | Particle size | Pore size | Molecular range |
| Unix-C SEC-300 | 1.8 μm | 300 Å | 5,000 - 1,250,000 Da |
Applications
Analysis of MAb on SRT®-C 300
Determination of MAb with SRT-C300.
Test Conditions:
Column: SRT®-C 300 (5 μm, 300 Å, 7.8x300 mm)
Mobile Phase: 150 mM sodium phosphate buffer, pH 7
Temperature: ambient (~23° C)
Flow rate: 1.0 mL/min
Detection: UV 214 nm
Concentration: 5 mg/mL
Injection volume: 2.5 μL
Sample: MAb
MAb321 Separation on Unix-C SEC-300
Seperation of MAb321 on Unix-C SEC-300.
Test Conditions:
Column: Unix-C SEC-300 (1.8 μm, 300 Å, 4.6 x 300 mm)
Mobile Phase: 150 mM phosphate buffer
Flow rate: 0.3 mL/min
System: UHPLC
Detection: UV 280 nm
Column temperature: ambient
Injection volume: 2μL
Sample: 5mg/ml MAb321
Downloads
Brochures
Sepax SRT 1010c SEC Life-Science Brochure
Sepax SRT SEC Life-Science Brochure
Unix-C SEC-300 UHPLC
Zenix-80
Applications
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Sepax SRT-C 300 Analysis of MAb SEC
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Sepax Unix-C SEC-300 MAb Separation
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Sepax Zenix-C SEC-300 Rituximab Analysis
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Sepax Zenix-C SEC Analysis of Insulin and HMW Aggregates
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Sepax Fusion Protein Separation on SEC & SAX
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Sepax Nanofilm SEC SRT-C Membran Protein Separation
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Sepax Proteomix SRT-SEC Zenix-C Zenix Complex Protein Sample Mixtures Analysis
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Sepax Zenix SEC-80 Zenix-C SEC-300 Pegylated Exenatide
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Sepax Zenix SEC, Zenix-C, Proteomix RP, Proteomix SAX Oligonucleotide Separation
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Sepax Zenix SEC and Proteomix Fusion Protein Separation on SEC and SAX
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sepax_sec_screening_kit
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Sepax Zenix SEC-80 Zenix-C SEC-80 Peptide Separation
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Sepax Zenix-C SEC-100 Short Oligonucleotides Analysis
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