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Proteomix
Proteomix Ion-exchange columns are especially designed for the separation of proteins, oligonucleotides, carbohydrates and peptides. The particles are made of highly cross-linked, non-porous polystyrene divinylbenzene. The surface of the particles is grafted with a hydrophilic, neutral polymer layer and ion-exchange groups are bound on this layer.
Proteomix HIC columns are specially designed for the separation of proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), oligonucleotides and peptides via the hydrophobic interaction chromatography (HIC) mechanism. The resin is made of non-porous polystyrene divinylbenzene (PS/DVB) and is modified with alkyl groups.
Proteomix RP columns are made of porous polystyrene divinylbenzene (PS/DVB). There are phenyl and substituted phenyl groups on the surface which enable hydrophobic interaction.
Technical Data
Proteomix ion exchange
| Phase | Particle size | pH range |
| Proteomix SCX-NP | 1.7, 3, 5, 10 μm | 2.0-12 |
| Proteomix WCX-NP | 1.7, 3, 5, 10 μm | 2.0-12 |
| Proteomix SAX-NP | 1.7, 3, 5, 10 μm | 2.0-12 |
| Proteomix WAX-NP | 1.7, 3, 5, 10 μm | 2.0-12 |
Proteomix HIC
| Phase | Modification | Particle size | pH range | Tmax |
| Proteomix HIC Etdyl | C2 | 1.7, 5, 10 μm | 2.0-12 | 80 °C |
| Proteomix HIC Propyl | C3 | 5, 10 μm | 2.0-12 | 80 °C |
| Proteomix HIC Butyl | C4 | 1.7, 5, 10 μm | 2.0-12 | 80 °C |
| Proteomix HIC Phenyl | Ph | 5, 10 μm | 2.0-12 | 80 °C |
Proteomix RP
| Phase | Modification | Particle size | Pore size | pH range | Tmax |
| Proteomix RP | Ph, substituted Ph | 5, 10 μm | 300, 500, 1000 Å | 1.0-14 | 80 °C |
Applications
mAb321/Erbitux/Rituximab at 80 ℃
Determination of Ertibux, Rituximab and mAb 321 with Proteomix ® RP -1000.
Test Conditions:
Column: Proteomix ® RP -1000, 5 μm, 1000 Å, 2.1 x 150 mm
Mobile Phase A: H2O + 0.1% TFA
Mobile Phase B: ACN + 0.1% TFA
Flow rate: 0.25 mL/min
Gradient: 23- 70% B in 30 min
Detection: UV 214 nm
Column temperature: 80°C
Injection volume: 2.5 μL
Sample: monoclonal antibody, 1mg/mL diluted with 0.1% TFA
RituximAb separation - NP5 and 1.7 µm
Abtrennung von RituximAb with NP5 und 1.7 µm.
Test Conditions:
Columns: Proteomix® HIC Butyl NP1.7 μm (1.7 μm, 4.6 x 100 mm)
Proteomix® HIC Butyl-NP5 μm (5 μm, 4.6 x 100 mm)
Mobile Phase A: 100 mM Natriumphosphatpuffer, 2 M Ammoniumsulfat, pH 7.0
Mobile Phase B: 100 mM Natriumphosphatpuffer, pH 7.0
Flow rate: 0.8 mL/min
Detection: UV 214 nm
Column temperature: 25°C
Downloads
Brochures
Proteomix® HIC Phases
Sepax Proteomix HPLC Life-Science Brochure
Sepax Proteomix RP HPLC Life-Science Brochure
Applications
Sepax Proteomix HIC-Butyl-1.7 Intact & Oxidized MAb Analysis
Sepax Proteomix HIC-Butyl-NP5 Erbitux & Rituximab MAb Separation
Sepax Proteomix RP-1000 Column Temperature-Effect-on-MAb Separation
Sepax Proteomix RP-1000 Proteomix RP-500 MAb Fragment
Sepax Proteomix SAX-NP10 Analysis of Glycoprotein
Sepax Proteomix SAX Fast Analysis of N-Linked Sialylated Glycan
Sepax Proteomix SAX Intact Glycoprotein Isoforms Separation
Sepax Proteomix SCX-NP5 MAb Characterization
Sepax Proteomix SCX-NP5 MAb-IgG1 & IgG2 Charge Variants Separation
Sepax Proteomix SRT-SEC Zenix-C Zenix Complex Protein Sample Mixtures Analysis
Sepax Proteomix RP-300 Separation of DNA
Sepax Bio-C18, Proteomix SAX Oligonucleotide Analysis
Sepax Antibodix WCX-NP5 Proteomix SCX-NP5 Ion Exchange Kit for MAb-separation
Sepax Fusion Protein Separation on SEC & SAX
Technical support
Sepax Proteomix User Manual
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