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Insulin
Insulin is a peptide hormone which, among other things, regulates the absorption of sugar molecules in body cells. Insulin stimulates body cells to absorb glucose from the blood - it therefore has a blood sugar-lowering effect. The natural antagonist of insulin is glucagon, which is responsible for increasing blood sugar levels in the body.
Insulin is a polypeptide made up of a total of 51 amino acids. The molecular mass is around 5800 Daltons.
The liquid chromatographic analysis of insulin is often carried out using size exclusion or reversed phase chromatography. Below you will find some columns and application examples from various manufacturers.
Applications
Reversed Phase Separation of Peptides with Nouryon Kromasil 100-5-C8
Peak identities
1. oxytocin 2. bradykinin 3. methionine enkephalin 4. angiotensin II 5. leucine enkephalin 6. angiotensin I 7. insulin 8. lysozyme 9. melittin
Test conditions
Column: Kromasil 100-5-C8 250x4.6mm (M05CMA25)
Mobile phase A: 10/90/0.1 - acetonitrile/water/TFA
Mobile phase B: 90/10/0.1 - acetonitrile/water/TFA
Gradient: 0 min 0%B, 8 min 25%B, 20 min 75%B
Flow rate: 2 mL/min
Detection: UV, 254 nm
Size Exclusion Chromatography of Insulin and Aggregates with Shodex Protein KW-802.5
Peak identities
1. high molecular weight proteins 2. insulin trimer 3. insulin dimer 4. insulin monomer
Test conditions
Column: Proetin KW-802.5 300x8mm
Mobile phase: 0.1 wt% L-Arginine/Acetonitrile/Acetic acid = 13/4/3
Flow rate: 0.5 mL/min
Detection: UV, 276 nm
Temperature: 25 °C
Downloads
Manufacturer overview
AMT
UHPLC - Reversed Phase:
- HALO Peptide ES-C18, 2µm
GL Sciences
HPLC - Reversed Phase:
- Inertsil WP300 C18, 5µm
HPLC - SEC:
- Inertsil WP300 Diol, 5µm
Nouryon - Kromasil
HPLC - Reversed Phase:
- Kromasil 100 C8, 5µm
Sepax
HPLC - SEC:
- Zenix-C SEC-80, 3µm
- Zenix-C SEC-150, 3µm
Shodex
HPLC - Reversed Phase:
- Asahipak C8P-50 4D, 5µm
HPLC - SEC:
- Protein KW802.5, 5µm
Thermo Fisher
HPLC - Reversed Phase:
- Accucore C18, 2.6µm
Tosoh
HPLC - Reversed Phase:
- TSKgel Octadecyl-NPR
HPLC - SEC:
- TSKgel Super SW2000, 4µm
HPLC - HILIC:
- TSKgel Amide-80
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