HALO 400 A Diphenyl, 3.4 µm, 0.5 x 150 mm

The HALO 400Å Diphenyl HPLC column 150x0.5mm, 3.4µm, has two phenyl units on a core-shell particle that are directly bonded to the silane. This results primarily in dipole-dipole and π-π interactions, as well as weaker hydrophobic interactions. This makes this phase suitable for reverse-phase chromatography. These various interactions of the HALO 400Å Diphenyl result in alternative selectivities compared to a C18 or C4 phase. The large pores of 400Å enable the analysis of large proteins and antibodies (mAb), such as denosumab, IgG2, or trastuzumab.

Features and Benefits

• Core-shell particles for fast methods
• UHPLC performance on HPLC systems
• High efficiency
• Low backpressure

Additional Notes

The HALO Bioclass series is based on a core-shell material. This means that the particles have a non-porous core and a porous shell. This allows for high efficiency while maintaining low backpressure. As a result, UHPLC performance is possible using a standard HPLC system. Compared to the HALO series, the HALO Bioclass series features larger pores (160 Å, 400 Å, and 1,000 Å). This allows biological samples—ranging from small peptides to proteins and antibodies—to be analyzed using core-shell technology. The service life can be extended by using appropriate precolumn cartridges.

Legal Notices

HALO is a registered trademark of the manufacturer Advanced Materials Technology, Inc., Wilmington, USA.