Inertsil ODS-EP 5µm, HPLC-Column 150x3.9mm HPLC Column

The Inertsil ODS-EP HPLC column 150x3.9mm, 5µm, features an octadecyl modification (also known as: ODS, RP-18, C18) with an embedded polar group between the silica surface and the C18 ligand. This polar embedding improves the wettability of the phase and allows for use with 100% aqueous eluents without the typical phase collapse. Inertsil ODS-EP is used in reverse-phase chromatography and offers an alternative selectivity to conventional C18 phases. Typical applications include polar active ingredients, LC/MS methods with aqueous or acidified eluents, acidic and basic analytes, and metabolite analysis.

Performance Characteristics and Benefits

• High-purity silica
• Controlled pore structure and reproducible surface
• High inertness due to reduced silanol and metal activity
• Excellent peak shapes for acidic, neutral, and basic analytes
• Reliable reproducibility for routine analysis

Additional Notes

The Inertsil series is based on a high-purity, spherical silica matrix whose pore structure, particle size distribution, and surface are carefully controlled during production. Low metal contamination and careful surface treatment reduce the activity of free silanol groups. This minimizes undesirable secondary interactions—particularly tailing and adsorption with basic or chelating analytes—and promotes stable retention times. This reproducible silica matrix forms the basis for the various Inertsil modifications and ensures reliable selectivities across the series. Appropriate Inertsil pre-column cartridges should be used to protect the analytical packing bed from particles and the sample matrix and to extend column lifespan.

Legal Notices

Inertsil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.