Inertsil C4 5µm, HPLC-Column 125x4.0mm HPLC Column

The Inertsil C4 HPLC column 125x4.0mm, 5µm, is butyl-modified and is used in reverse-phase chromatography. The short C4 chain produces weaker hydrophobic interactions than C8 or C18 phases. Inertsil C4 is therefore suitable for analytes that retain too long on more hydrophobic phases. Typical applications include fast RP separations, highly hydrophobic substances, peptides and proteins with high affinity for longer alkyl chains, as well as methods requiring mild hydrophobic selectivity.

Performance Characteristics and Benefits

• High-purity silica
• Controlled pore structure and reproducible surface
• High inertness due to reduced silanol and metal activity
• Excellent peak shapes for acidic, neutral, and basic analytes
• Reliable reproducibility for routine analysis

Additional Notes

The Inertsil series is based on a high-purity, spherical silica matrix whose pore structure, particle size distribution, and surface are carefully controlled during production. Low metal contamination and careful surface treatment reduce the activity of free silanol groups. This minimizes undesirable secondary interactions—particularly tailing and adsorption with basic or chelating analytes—and promotes stable retention times. This reproducible silica matrix forms the basis for the various Inertsil modifications and ensures reliable selectivities across the series. Appropriate Inertsil pre-column cartridges should be used to protect the analytical packing bed from particles and the sample matrix and to extend column lifespan.

Legal Notices

Inertsil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.