Inertsil WP300-Diol Preparative column 50.0 x 250mm HPLC Column

The Inertsil WP300 Diol HPLC column 250x50mm, 5µm, features dihydroxypropyl groups on wide-pore silica with a pore size of 300 Å. Inertsil WP300 Diol can be used for aqueous and organic size-exclusion chromatography (SEC/GFC) and is designed for large molecules. In addition, the diol surface also enables normal-phase and selected reverse-phase applications. Typical applications include proteins, biopolymers, PEG-conjugated compounds, liposomes, large natural products, and molecular-size-dependent separations.

Performance Characteristics and Benefits

• High-purity silica
• Controlled pore structure and reproducible surface
• High inertness due to reduced silanol and metal activity
• Excellent peak shapes for acidic, neutral, and basic analytes
• Reliable reproducibility for routine analysis

Additional Notes

The Inertsil series is based on a high-purity, spherical silica matrix whose pore structure, particle size distribution, and surface are carefully controlled during production. Low metal contamination and careful surface treatment reduce the activity of free silanol groups. This minimizes undesirable secondary interactions—particularly tailing and adsorption with basic or chelating analytes—and promotes stable retention times. This reproducible silica matrix forms the basis for the various Inertsil modifications and ensures reliable selectivities across the series. Appropriate Inertsil pre-column cartridges should be used to protect the analytical packing bed from particles and the sample matrix and to extend column lifespan.

Legal Notices

Inertsil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.