ProteoSil 100-SEC Analytical Column 5um, 7.6x250mm SEC / GPC Columns

The ProteoSil 100-SEC HPLC column 250x7.6mm, 5µm, is based on a diol modification with a pore size of 100 Å for size-exclusion chromatography. Unlike RP phases, the ProteoSil 100-SEC is not based on hydrophobic retention, but rather on the analytes’ access to the pore system. The 100 Å pores are suitable for smaller proteins, peptides, oligonucleotides, and nucleic acids with a molecular weight below approximately 5,000 Da. This SEC/GFC phase can be used in aqueous buffer systems and is of interest for methods in which molecular size, low-molecular-weight components, or smaller fragments are the primary focus.

Features and Benefits

• Bio-LC column series for proteins, peptides, oligonucleotides, nucleic acids, and biopharmaceutical applications
• High-purity silica with controlled pore sizes of 100 Å, 200 Å, and 300 Å
• Choice between reverse-phase, HILIC, and size-exclusion chromatography
• Stainless steel, PEEK, and bio-inert hardware for various requirements
• Low lot-to-lot variability and consistent quality, even for LC/MS methods

Additional Information

The ProteoSil series is designed for bioseparations in which pore size, modification, and hardware must be specifically tailored to sensitive biomolecules. It is based on a high-purity silica matrix with pore sizes of 100 Å, 200 Å, or 300 Å. Smaller pores are particularly suitable for low-molecular-weight peptides, while 200 Å materials are used for medium-sized peptides and oligonucleotides. For larger proteins, hydrophobic peptides, and antibodies, 300 Å materials are available. In addition, ProteoSil HILIC and ProteoSil SEC round out the series for hydrophilic analytes and size-based separations. Using appropriate ProteoSil pre-column cartridges or guard columns, the analytical column can be protected from particles, strongly adsorbing sample components, and matrix carryover.

Legal Information

ProteoSil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.