Chiralpak T101 20µm 250x4.6mm HPLC Column

The Chiralpak T101 HPLC column 250x4.6mm, 20µm, is a coated, specialised chiral phase on 20 µm silica. The modification of Chiralpak T101 is described as a polymeric, cellulose-derived chiral selector that is physically applied to the silica support. Chiralpak T101 is therefore not an immobilised phase; its coated design can offer high loading capacity and pronounced enantioselectivity, but requires careful selection of the solvent. This phase is of particular interest for HPLC and SFC applications in preparative method development and scale-up. Chiralpak T101 can be used when specific cellulose-based selectivity is required and conventional standard phases such as AD, AS, OD or OJ do not provide the desired separation. Typical applications include preparative enantiomer separation, method development for racemates and the optimisation of larger-scale separations.

Performance Characteristics and Advantages

• Amylose- and cellulose-based polysaccharide phases with high enantioselectivity
• Immobilized Chiralpak phases with very broad solvent compatibility
• Coated Chiralpak phases with proven selectivity and good loading capacity
• Suitable for use in normal-phase HPLC, reverse-phase HPLC, polar-organic HPLC, and SFC
• Wide selection of complementary selectors for efficient chiral screening
• Specialized phases for amino acids, peptides, chiral acids, and ligand exchange methods

Additional Notes

Chiralpak includes both amylose-based and cellulose-based polysaccharide phases. In the immobilized phases, the chiral selector is fixed to the silica support. This allows for the use of significantly more organic solvents than with coated phases, which is particularly helpful when samples have limited solubility. Coated Chiralpak phases are based on polysaccharide derivatives physically applied to silica. They have a long history of use and often offer very high selectivity and good loading capacity. The most important limitation is solvent compatibility: mobile phases or sample solutions that can dissolve the polysaccharide layer must be avoided. Immobilized and coated variants with similar modifications are not necessarily chromatographically interchangeable. Immobilization increases robustness and solvent flexibility but can alter the spatial accessibility of the selector. Therefore, methods should be reviewed when switching between coated and immobilized phases and reoptimized as needed.

Legal Notices

Chiralpak is a registered trademark of Daicel Corporation, Osaka, Japan.