Chiralpak DSA 5µm 50x2.0mm HPLC Column

The Chiralpak DSA HPLC column 50x2.0mm, 5µm, based on dog serum albumin (DSA) as an immobilised protein selector. Chiralpak DSA therefore does not have an amylose or cellulose base, but rather serum albumin immobilised on spherical 5-µm silica particles. The advantage lies in the specific investigation of albumin-dependent binding and retention patterns under mild aqueous RP-HPLC conditions, whilst the protein matrix dictates a narrower operating window than robust synthetic phases. Chiralpak DSA is primarily used for comparative drug-protein binding studies, species comparisons and the assessment of drug interactions with dog serum albumin. Chiralpak DSA selectivity can therefore provide additional information when HSA or BSA data are insufficient.

Performance Characteristics and Advantages

• Protein-based chiral selectors for enantioselective reverse-phase HPLC
• Immobilized protein phases on spherical 5-µm silica
• Compatible with aqueous buffers, low levels of organic modifiers, and moderate pH values
• Enantioselectivity can be specifically optimized via pH, buffer type, and organic modifier
• Complementary selectors such as AGP, CBH, HSA, and animal serum albumin variants
• Suitable for drug discovery, purity testing, environmental analysis, and drug-protein binding studies

Additional Notes

Protein-based Chiralpak materials differ fundamentally from traditional amylose- or cellulose-based polysaccharide phases. The chiral selector is a protein immobilized on spherical silica particles. This creates complex binding sites where ionic interactions, hydrophobic interactions, and hydrogen bonds can contribute to enantiomer recognition. Since the protein selectors are immobilized, these are not merely coated surfaces. The advantage of immobilization lies in a more stable fixation of the selector during standard aqueous reverse-phase operation and in highly reproducible separation conditions. The disadvantage compared to more robust synthetic or polysaccharide-based immobilized phases is the narrower operating window: high concentrations of organic solvents, strongly denaturing solvents, extreme pH values, high temperatures, and excessively high flow rates should be avoided. The selectivity of protein-based Chiralpak columns can be controlled primarily by pH, buffer composition, and the type and concentration of the organic modifier. These mild, aqueous conditions make the phases particularly suitable for chiral active ingredients, bioanalytical applications, and protein binding studies; however, they require careful column maintenance and the use of appropriate sample solutions.

Legal Notices

Chiralpak is a registered trademark of Daicel Corporation, Osaka, Japan.