Chiralpak HSA 5µm 100x2.0mm HPLC Column

The Chiralpak HSA HPLC column 100x2.0mm, 5µm, is based on human serum albumin (HSA) as an immobilised protein selector on spherical 5-µm silica. The advantage is the direct use of albumin-like binding sites for chiral separations and drug-protein binding studies; One limitation is that mild reverse-phase conditions must be maintained, using suitable buffers and a controlled organic modifier. Chiralpak HSA is particularly suitable for weak and strong acids, zwitterionic compounds and non-protolytic substances. Typical applications include active pharmaceutical ingredients, bioanalytics, drug binding to human serum albumin, and method development under aqueous RP-HPLC conditions.

Performance Characteristics and Advantages

• Protein-based chiral selectors for enantioselective reverse-phase HPLC
• Immobilized protein phases on spherical 5-µm silica
• Compatible with aqueous buffers, low levels of organic modifiers, and moderate pH values
• Enantioselectivity can be specifically optimized via pH, buffer type, and organic modifier
• Complementary selectors such as AGP, CBH, HSA, and animal serum albumin variants
• Suitable for drug discovery, purity testing, environmental analysis, and drug-protein binding studies

Additional Notes

Protein-based Chiralpak materials differ fundamentally from traditional amylose- or cellulose-based polysaccharide phases. The chiral selector is a protein immobilized on spherical silica particles. This creates complex binding sites where ionic interactions, hydrophobic interactions, and hydrogen bonds can contribute to enantiomer recognition. Since the protein selectors are immobilized, these are not merely coated surfaces. The advantage of immobilization lies in a more stable fixation of the selector during standard aqueous reverse-phase operation and in highly reproducible separation conditions. The disadvantage compared to more robust synthetic or polysaccharide-based immobilized phases is the narrower operating window: high concentrations of organic solvents, strongly denaturing solvents, extreme pH values, high temperatures, and excessively high flow rates should be avoided. The selectivity of protein-based Chiralpak columns can be controlled primarily by pH, buffer composition, and the type and concentration of the organic modifier. These mild, aqueous conditions make the phases particularly suitable for chiral active ingredients, bioanalytical applications, and protein binding studies; however, they require careful column maintenance and the use of appropriate sample solutions.

Legal Notices

Chiralpak is a registered trademark of Daicel Corporation, Osaka, Japan.