Chiralpak QN-AX 5µm 150x4.6mm HPLC Column

The Chiralpak QN-AX HPLC/SFC column 150x4.6mm, 5µm; it is not based on an amylose or cellulose backbone. The specific modifier used is O-9-(tert-butylcarbamoyl)quinine, which acts as a weakly anion-exchange chiral selector. Chiralpak QN-AX is not a coated or immobilised amylose/cellulose polysaccharide phase, but a specialised phase designed for specific ionic or ligand-exchange mechanisms. Chiralpak QN-AX is used in polar-organic HPLC and SFC and is particularly suitable for chiral acids, N-derivatised amino acids, phenols and acidic drug candidates. Chiralpak QN-AX utilises ionic interactions with anions; neutral substances without the appropriate functionality benefit less from this. When developing methods using Chiralpak QN-AX, the pH, additives, counter-ions and solvents should therefore be specifically tailored to the dominant interaction mechanism.

Performance Characteristics and Advantages

• Amylose- and cellulose-based polysaccharide phases with high enantioselectivity
• Immobilized Chiralpak phases with very broad solvent compatibility
• Coated Chiralpak phases with proven selectivity and good loading capacity
• Suitable for use in normal-phase HPLC, reverse-phase HPLC, polar-organic HPLC, and SFC
• Wide selection of complementary selectors for efficient chiral screening
• Specialized phases for amino acids, peptides, chiral acids, and ligand exchange methods

Additional Notes

Chiralpak includes both amylose-based and cellulose-based polysaccharide phases. In the immobilized phases, the chiral selector is fixed to the silica support. This allows for the use of significantly more organic solvents than with coated phases, which is particularly helpful when samples have limited solubility. Coated Chiralpak phases are based on polysaccharide derivatives physically applied to silica. They have a long history of use and often offer very high selectivity and good loading capacity. The most important limitation is solvent compatibility: mobile phases or sample solutions that can dissolve the polysaccharide layer must be avoided. Immobilized and coated variants with similar modifications are not necessarily chromatographically interchangeable. Immobilization increases robustness and solvent flexibility but can alter the spatial accessibility of the selector. Therefore, methods should be reviewed when switching between coated and immobilized phases and reoptimized as needed.

Legal Notices

Chiralpak is a registered trademark of Daicel Corporation, Osaka, Japan.